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Literature summary extracted from

  • Berman, T.; Magasanik, B.
    The pathway of myo-inositol degradation in Aerobacter aerogenes. Dehydrogenation and dehydration (1966), J. Biol. Chem., 241, 800-806.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.18 2-keto-myo-inositol
-
 Klebsiella aerogenes
4.2.1.44 EDTA inhibition can be alleviated by either Mn2+ or Co2+ Klebsiella aerogenes
4.2.1.44 p-hydroxymercuribenzoate on addition of glutathione or mercaptoethanol the activity is regained Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.44 0.15
-
 2-keto-myo-inositol
-
 Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.44 Co2+ increases activity Klebsiella aerogenes
4.2.1.44 Mn2+ increases activity Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.18 D-2,3-diketo-4-deoxy-epi-inositol + NADH Klebsiella aerogenes
-
 ketodeoxyinositol + NAD+
-
 ?
1.1.1.18 myo-inositol + NAD+ Klebsiella aerogenes
-
 2,4,6/3,5-pentahydroxycyclohexanone + NADH
-
 ?
4.2.1.44 (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone Klebsiella aerogenes pathway of myo-inositol degradation (3R,4S,5R)-3,4,5-trihydroxycyclohexane-1,2-dione + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.18 Klebsiella aerogenes
-
-
-
4.2.1.44 Klebsiella aerogenes
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.1.44 unstable when exposed to oxygen Klebsiella aerogenes

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.18 partial Klebsiella aerogenes
4.2.1.44 partial Klebsiella aerogenes

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.44 2,4,6/3,5-Pentahydroxycyclohexanone = 3,5/4-trihydroxycyclohexa-1,2-dione + H2O mechanism Klebsiella aerogenes
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.18 cell culture
-
 Klebsiella aerogenes
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.18 280
-
-
 Klebsiella aerogenes
4.2.1.44 270
-
-
 Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
1.1.1.18 -10°C, several months Klebsiella aerogenes
4.2.1.44 -10°C, nitrogen atmosphere, for 1 month, about 50% loss of activity Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.18 D-2,3-diketo-4-deoxy-epi-inositol + NADH
-
 Klebsiella aerogenes ketodeoxyinositol + NAD+
-
 ?
1.1.1.18 myo-inositol + NAD+
-
 Klebsiella aerogenes 2,4,6/3,5-pentahydroxycyclohexanone + NADH
-
 ?
4.2.1.44 (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone
-
 Klebsiella aerogenes (3R,4S,5R)-3,4,5-trihydroxycyclohexane-1,2-dione + H2O
-
 ?
4.2.1.44 (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone pathway of myo-inositol degradation Klebsiella aerogenes (3R,4S,5R)-3,4,5-trihydroxycyclohexane-1,2-dione + H2O
-
 ?
4.2.1.44 L-2-Keto-epi-inositol
-
 Klebsiella aerogenes ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.44 6 6.2 2-keto-myo-inositol Klebsiella aerogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.18 NAD+ preferred hydrogen acceptor Klebsiella aerogenes
1.1.1.18 NADH
-
 Klebsiella aerogenes