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Literature summary extracted from

  • Yamada H.; Nagao, A.; Nishise, H.; Tani, Y.
    Glycerol dehydrogenase from Cellulomonas sp. NT 3060: purification and characterization (1982), Agric. Biol. Chem., 46, 2333-2339.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.6 NH4+ oxidation and reduction greatly increased Cellulomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.6 1,10-phenanthroline 1 mM Cellulomonas sp.
1.1.1.6 Cd2+ strongly inhibitory Cellulomonas sp.
1.1.1.6 Cu2+ strong inhibition Cellulomonas sp.
1.1.1.6 dihydroxyacetone above 0.5 mM Cellulomonas sp.
1.1.1.6 Fe3+
-
 Cellulomonas sp.
1.1.1.6 K+ reduction of dihydroxyacetone Cellulomonas sp.
1.1.1.6 p-chloromercuribenzoate
-
 Cellulomonas sp.
1.1.1.6 sodium tetraborate 1 mM, 53% inhibition Cellulomonas sp.
1.1.1.6 Zn2+ strong inhibitory Cellulomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.6 0.089
-
 NAD+ pH 9.0, 25°C Cellulomonas sp.
1.1.1.6 0.385
-
 dihydroxyacetone pH 6.0, 25°C Cellulomonas sp.
1.1.1.6 10.9
-
 glycerol pH 9.0, 25°C Cellulomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.6 cytoplasm soluble, isolation from crude cell extract Cellulomonas sp. 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.6 K+ oxidation of glycerol os greatly increased Cellulomonas sp.
1.1.1.6 Li+ oxidation slightly increased Cellulomonas sp.
1.1.1.6 Na+ oxidation and reduction slightly increased Cellulomonas sp.
1.1.1.6 Rb+ oxidation greatly increased Cellulomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.6 glycerol + NAD+ Cellulomonas sp.
-
 dihydroxyacetone + NADH + H+
-
 ?
1.1.1.6 glycerol + NAD+ Cellulomonas sp. NT3060
-
 dihydroxyacetone + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.6 Cellulomonas sp.
-
-
-
1.1.1.6 Cellulomonas sp. NT3060
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.6
-
 Cellulomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.6 138
-
-
 Cellulomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.6 1,2-propanediol + NAD+ relative activity is 116% compared to oxidation of glycerol Cellulomonas sp. hydroxyacetone + NADH
-
 ?
1.1.1.6 1,2-propanediol + NAD+ relative activity is 116% compared to oxidation of glycerol Cellulomonas sp. NT3060 hydroxyacetone + NADH
-
 ?
1.1.1.6 1,3-butanediol + NAD+ relative activity is 6.5% compared to oxidation of glycerol Cellulomonas sp. ?
-
 ?
1.1.1.6 1,3-propanediol + NAD+ relative rate of oxidation is 18% compared to oxidation of glycerol Cellulomonas sp. ?
-
 ?
1.1.1.6 1,4-butanediol + NAD+ relative activity is 0.3% compared to oxidation of glycerol Cellulomonas sp. ?
-
 ?
1.1.1.6 2,3-butanediol + NAD+
-
 Cellulomonas sp. 3-hydroxybutane-2-one + NADH
-
 ?
1.1.1.6 2,3-butanediol + NAD+
-
 Cellulomonas sp. NT3060 3-hydroxybutane-2-one + NADH
-
 ?
1.1.1.6 DL-alpha-glycerophosphate + NAD+ oxidation at 16% compared to oxidation of glycerol Cellulomonas sp. ?
-
 ?
1.1.1.6 DL-glyceraldehyde + NAD+
-
 Cellulomonas sp. 3-hydroxypyruvaldehyde + NADH
-
 ?
1.1.1.6 ethanediol + NAD+
-
 Cellulomonas sp. glycolaldehyde + NADH reduction at concentration of 50 mM r
1.1.1.6 ethanediol + NAD+
-
 Cellulomonas sp. NT3060 glycolaldehyde + NADH reduction at concentration of 50 mM r
1.1.1.6 ethylene glycol + NAD+
-
 Cellulomonas sp. ?
-
 ?
1.1.1.6 glycerol + NAD+
-
 Cellulomonas sp. dihydroxyacetone + NADH + H+
-
 ?
1.1.1.6 glycerol + NAD+
-
 Cellulomonas sp. dihydroxyacetone + NADH + H+
-
 r
1.1.1.6 glycerol + NAD+
-
 Cellulomonas sp. NT3060 dihydroxyacetone + NADH + H+
-
 ?
1.1.1.6 glycerol + NAD+
-
 Cellulomonas sp. NT3060 dihydroxyacetone + NADH + H+
-
 r
1.1.1.6 glycerol-alpha-monochlorohydrin + NAD+ relative rate of oxidation is 71% compared to oxidation of glycerol Cellulomonas sp. ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.6 50
-
 oxidation of glycerol Cellulomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.6 30
-
 60 min, stable in the pH range pH 5.5-10 Cellulomonas sp.
1.1.1.6 50
-
 15 min, stable around pH 7.0 Cellulomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.6 6
-
 reaction of dihydroxyacetone Cellulomonas sp.
1.1.1.6 9
-
 oxidation of glycerol Cellulomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.6 4.8 7.8 pH 4.8: about 40% of maximal activity, pH 7.8: about 50% of maximal activity, reduction of dihydroxyacetone Cellulomonas sp.
1.1.1.6 7 10 pH 7.0: about 60% of maximal activity, pH 10.0: about 85% of maximal activity, oxidation of glycerol Cellulomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.6 5.5 10 30°C, 60 min, stable Cellulomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.6 NAD+
-
 Cellulomonas sp.
1.1.1.6 NADH
-
 Cellulomonas sp.