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Literature summary extracted from

  • Straganz, G.D.; Glieder, A.; Brecker, L.; Ribbons, D.W.; Steiner, W.
    Acetylacetone-cleaving enzyme Dke1: a novel C-C-bond-cleaving enzyme from Acinetobacter johnsonii (2003), Biochem. J., 369, 573-581.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.50
-
 Acinetobacter johnsonii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.50 EDTA largely irreversible losses Acinetobacter johnsonii
1.13.11.50 H2O2 0.1 mM, immediate and total inhibition Acinetobacter johnsonii
1.13.11.50 hexacyanoferrate(III) 2.5 mM, 80% decrease in activity within 10 min Acinetobacter johnsonii
1.13.11.50 KCN largely irreversible losses Acinetobacter johnsonii
1.13.11.50 o-phenanthroline largely irreversible losses Acinetobacter johnsonii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.50 0.000009
-
 Pentane-2,4-dione
-
 Acinetobacter johnsonii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.50 Fe2+ 0.9-1.0 iron atoms per subunit, bound to active enzyme, essential for catalytic activity Acinetobacter johnsonii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.50 65000
-
 gel filtration Acinetobacter johnsonii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.50 pentane-2,4-dione + O2 Acinetobacter johnsonii
-
 acetate + 2-oxopropanal
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.50 Acinetobacter johnsonii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.50
-
 Acinetobacter johnsonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.50 2,4-dioxopentanoic acid ethyl ester + O2
-
 Acinetobacter johnsonii ?
-
 ?
1.13.11.50 2,4-octanedione + O2
-
 Acinetobacter johnsonii ?
-
 ?
1.13.11.50 2-acetylcyclohexanone + O2
-
 Acinetobacter johnsonii ?
-
 ?
1.13.11.50 3,5-heptanedione + O2
-
 Acinetobacter johnsonii ?
-
 ?
1.13.11.50 3-methyl-2,4-pentanedione + O2
-
 Acinetobacter johnsonii ?
-
 ?
1.13.11.50 3-oxobutanone + O2
-
 Acinetobacter johnsonii ?
-
 ?
1.13.11.50 4-hydroxy-4-methyl-2-pentanone + O2
-
 Acinetobacter johnsonii ?
-
 ?
1.13.11.50 ethylacetoacetate + O2
-
 Acinetobacter johnsonii ?
-
 ?
1.13.11.50 additional information no substrate: 4-hydroxy-4-methyl-2-pentanone, 3-oxobutanoate, quercetin, catechols, 2,4`-dihydroxyacetophenone, ascorbate Acinetobacter johnsonii ?
-
 ?
1.13.11.50 pentane-2,4-dione + O2
-
 Acinetobacter johnsonii acetate + 2-oxopropanal
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.50 tetramer 4 x 16000, SDS-PAGE, MALDI-TOF MS Acinetobacter johnsonii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.50 6.5 8.5
-
 Acinetobacter johnsonii