EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.57 | 0.54 | - |
2-oxoglutarate | cosubstrate phenylalanine | Rattus norvegicus | |
2.6.1.57 | 7.7 | - |
L-phenylalanine | pH 8.0. 37°C | Rattus norvegicus | |
2.6.1.57 | 10 | - |
L-tryptophan | - |
Rattus norvegicus | |
2.6.1.57 | 11.8 | - |
L-tyrosine | pH 8.0. 37°C | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.6.1.57 | cytosol | - |
Rattus norvegicus | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.57 | 52000 | - |
2 * 52000, SDS-PAGE | Rattus norvegicus |
2.6.1.57 | 100000 | - |
gel filtration, sucrose density gradient centrifugation | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.57 | Rattus norvegicus | - |
vitamin B6-deficient strain | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.57 | vitamin B6-deficient strain, ammonium sulfate, DEAE-cellulose, isoelectric focusing, Sephacryl s-200, hydroxylapatite | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.6.1.57 | small intestine | mucosa | Rattus norvegicus | - |
2.6.1.57 | small intestine | not in muscle phase | Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.57 | 15.4 | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
2.6.1.57 | -20°C, at least 3 weeks, no loss of activity | Rattus norvegicus |
2.6.1.57 | 0-4°C, 2 weeks, little loss of activity | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.57 | 5-hydroxytryptophan + 2-oxoglutarate | 19.5% of the activity with phenylalanine | Rattus norvegicus | 3-(5-hydroxyindole)-2-oxopropanoate + L-glutamate | - |
? | |
2.6.1.57 | L-phenylalanine + 2-oxoglutarate | best amino acid substrate, specific for 2-oxoglutarate, little or no activity with glyoxylate, pyruvate, 2-oxo-4-methylthiobutyrate and alpha-ketoadipate | Rattus norvegicus | phenylpyruvate + L-glutamate | - |
r | |
2.6.1.57 | L-tryptophan + 2-oxoglutarate | 39.7% of activity with phenylalanine | Rattus norvegicus | 3-indole-2-oxopropanoate + L-glutamate | - |
? | |
2.6.1.57 | L-tyrosine + 2-oxoglutarate | 23.7% of activity with phenylalanine | Rattus norvegicus | p-hydroxyphenylpyruvate + L-glutamate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.57 | dimer | 2 * 52000, SDS-PAGE | Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.57 | 37 | - |
assay at | Rattus norvegicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.57 | 55 | - |
approx. 30% and 27% loss of activity after 10 min with L-tyrosine and L-phenylalanine, respectively, as subtrates | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.57 | 8 | - |
with phenylalanine | Rattus norvegicus |