EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.47 | pyridoxal | - |
Saccharomyces cerevisiae | |
2.5.1.47 | pyridoxal hydrochloride | 54% inhibition at 1 mM | Saccharomyces cerevisiae | |
2.5.1.49 | pyridoxal | inhibits the activity competitively with respect to pyridoxal 5'-phosphate | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.47 | O-acetyl-L-Ser + sulfide | Saccharomyces cerevisiae | functions as a Cys synthase rather than as a homocysteine synthase in vivo | L-Cys + acetate | - |
? | |
2.5.1.49 | O-acetyl-L-homoserine + H2S | Saccharomyces cerevisiae | - |
L-homocysteine + acetic acid | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
2.5.1.49 | urea | denatured by 2.0 M urea | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.47 | Saccharomyces cerevisiae | - |
- |
- |
2.5.1.49 | Saccharomyces cerevisiae | - |
baker's yeast | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.47 | - |
Saccharomyces cerevisiae |
2.5.1.49 | - |
Saccharomyces cerevisiae |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.5.1.47 | O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate | mechanism similar to those of other pyridoxal enzymes | Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.47 | 25.2 | - |
- |
Saccharomyces cerevisiae |
2.5.1.49 | 25.2 | - |
- |
Saccharomyces cerevisiae |
EC Number | Storage Stability | Organism |
---|---|---|
2.5.1.47 | -20 C, potassium phosphate buffer, pH 6.5, 1 mM EDTA, at least several days, no loss of activity | Saccharomyces cerevisiae |
2.5.1.49 | -20°C, can be stored without any loss of activity | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.47 | O-acetyl-L-Ser + sulfide | functions as a Cys synthase rather than as a homocysteine synthase in vivo | Saccharomyces cerevisiae | L-Cys + acetate | - |
? | |
2.5.1.49 | O-acetyl-L-homoserine + H2S | - |
Saccharomyces cerevisiae | L-homocysteine + acetic acid | - |
? | |
2.5.1.49 | O-acetyl-L-homoserine + methyl mercaptan | - |
Saccharomyces cerevisiae | L-methionine + acetic acid | - |
? | |
2.5.1.49 | O-acetyl-L-serine + H2S | - |
Saccharomyces cerevisiae | L-cysteine + acetic acid | - |
? | |
2.5.1.49 | O-acetyl-L-serine + methanethiol | reaction only in phosphate buffer, relative activity compared to O-acetyl-L-homoserine 14.8% | Saccharomyces cerevisiae | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.49 | OAH | - |
Saccharomyces cerevisiae |
2.5.1.49 | OAS-OAH sulfhydrylase | - |
Saccharomyces cerevisiae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.47 | additional information | - |
pyridoxal 5'-phosphate stabilizes against heat inactivation | Saccharomyces cerevisiae |
2.5.1.47 | 55 | - |
5 min, 45% loss of activity, without pyridoxal 5'-phosphate | Saccharomyces cerevisiae |
2.5.1.47 | 65 | - |
5 min, complete loss of activity, without pyridoxal 5'-phosphate | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.47 | pyridoxal 5'-phosphate | stoichiometry, binds to Lys, protects against inactivation by heat, urea, and trypsin, four binding sites per mol apoenzyme, association constant | Saccharomyces cerevisiae | |
2.5.1.49 | pyridoxal 5'-phosphate | - |
Saccharomyces cerevisiae |