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Literature summary extracted from

  • Ikegami, F.; Kaneko, M.; Lambein, F.; Kuo, Y.H.; Murakoshi, I.
    Difference between uracilylalanine synthases and cysteine synthases in Pisum sativum (1987), Phytochemistry, 26, 2699-2704.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.033
-
 H2S pH 7.8, 35°C Pisum sativum
2.5.1.47 0.038
-
 H2S pH 7.8, 35°C, isoenzyme 2 Pisum sativum
2.5.1.47 2.1
-
 O-acetyl-L-Ser pH 8.0, 30°C, isoenzyme 1 Pisum sativum
2.5.1.47 2.3
-
 O-acetyl-L-Ser pH 8.0, 30°C, isoenzyme 2 Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 26000
-
 2 * 26000, SDS-PAGE Pisum sativum
2.5.1.47 52000
-
 gel filtration Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Pisum sativum
-
 three isoforms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.47 seedling maximal activity Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 852
-
 isoenzyme 2 Pisum sativum
2.5.1.47 933
-
 isoenzyme 1 Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetyl-L-Ser + 2-propene-1-thiol 2.6% and 6.8% of the activity with sulfide, isoenzyme 1 and 2, respectively Pisum sativum S-allyl-L-cysteine + ?
-
 ?
2.5.1.47 O-acetyl-L-Ser + H2S
-
 Pisum sativum L-Cys + acetate
-
 ?
2.5.1.47 O-acetyl-L-Ser + mercaptoacetic acid 2.3% and 1.6% of activity with sulfide, isoenzymes 1 and 2, respectively Pisum sativum S-carboxymethyl-L-cysteine
-
 ?
2.5.1.47 O-acetyl-L-Ser + methyl mercaptan 4% and 1% of activity with sulfide, isoenzymes 1 and 2, respectively Pisum sativum S-methylcysteine + acetate
-
 ?
2.5.1.47 O-acetyl-L-serine
-
 Pisum sativum L-cysteine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 dimer 2 * 26000, SDS-PAGE Pisum sativum
2.5.1.47 More amino acid composition Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.47 pyridoxal 5'-phosphate
-
 Pisum sativum