EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.31 | cardiolipin | weak stimulation | Micrococcus luteus | |
2.5.1.31 | cardiolipin | Lactobacillus plantarum, Escherichia coli, and bovine cardiolipin. Good activator in absence of detergent | Lactiplantibacillus plantarum | |
2.5.1.31 | cetyl sulfate | half-maximal activation at 0.04 mM | Lactiplantibacillus plantarum | |
2.5.1.31 | deoxycholate | weak stimulation | Bacillus subtilis | |
2.5.1.31 | deoxycholate | weak stimulation | Micrococcus luteus | |
2.5.1.31 | deoxycholate | half-maximal activation at 0.33 mM | Lactiplantibacillus plantarum | |
2.5.1.31 | Dodecyl sulfate | half-maximal activation at 0.075 mM | Lactiplantibacillus plantarum | |
2.5.1.31 | lecithin | weak stimulation | Bacillus subtilis | |
2.5.1.31 | lecithin | from egg, stimulates | Micrococcus luteus | |
2.5.1.31 | oleic acid | good activator in absence of detergent | Lactiplantibacillus plantarum | |
2.5.1.31 | phosphatidic acid | from egg, good activator in absence of detergent | Lactiplantibacillus plantarum | |
2.5.1.31 | Triton X-100 | stimulates | Micrococcus luteus | |
2.5.1.31 | Triton X-100 | strong stimulation | Bacillus subtilis | |
2.5.1.31 | Triton X-100 | half-maximal activation at 3.4 mM | Lactiplantibacillus plantarum | |
2.5.1.31 | Tween 80 | strong stimulation | Bacillus subtilis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.31 | 1,2-Cyclohexanedione | - |
Lactiplantibacillus plantarum | |
2.5.1.31 | Butanedione | - |
Lactiplantibacillus plantarum | |
2.5.1.31 | iodoacetate | - |
Lactiplantibacillus plantarum | |
2.5.1.31 | additional information | no inhibition by phosphate | Lactiplantibacillus plantarum | |
2.5.1.31 | Phenylglyoxal | - |
Lactiplantibacillus plantarum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.31 | 0.00013 | - |
(E,E)-farnesyl diphosphate | pH 7.5, 35°C | Lactiplantibacillus plantarum | |
2.5.1.31 | 0.00192 | - |
isopentenyl diphosphate | pH 7.5, 35°C | Lactiplantibacillus plantarum | |
2.5.1.31 | 0.008 | - |
(E,E)-farnesyl diphosphate | - |
Micrococcus luteus | |
2.5.1.31 | 0.0091 | - |
(E,E)-farnesyl diphosphate | - |
Bacillus subtilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.31 | Co2+ | divalent cation required | Lactiplantibacillus plantarum | |
2.5.1.31 | Co2+ | divalent cation required | Bacillus subtilis | |
2.5.1.31 | Co2+ | divalent cation required | Micrococcus luteus | |
2.5.1.31 | Co2+ | enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+ | Micrococcus luteus | |
2.5.1.31 | Co2+ | enzyme is equally stimulated by 0.2 mM Mg2+, 0.2 mM Co2+ or 0.1 mM Mn2+ | Lactiplantibacillus plantarum | |
2.5.1.31 | K+ | stimulates in presence of Mg2+ | Lactiplantibacillus plantarum | |
2.5.1.31 | K+ | stimulates in presence of Mg2+ | Bacillus subtilis | |
2.5.1.31 | Mg2+ | divalent cation required | Lactiplantibacillus plantarum | |
2.5.1.31 | Mg2+ | divalent cation required | Bacillus subtilis | |
2.5.1.31 | Mg2+ | divalent cation required | Micrococcus luteus | |
2.5.1.31 | Mg2+ | enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+ | Micrococcus luteus | |
2.5.1.31 | Mg2+ | enzyme is equally stimulated by 0.2 mM Mg2+, 0.2 mM Co2+ or 0.1 mM Mn2+ | Lactiplantibacillus plantarum | |
2.5.1.31 | Mg2+ | best stimulation by Mg2+. Mn2+ and Zn2+ may partially replace Mn2+ | Bacillus subtilis | |
2.5.1.31 | Mn2+ | can partially replace Mg2+ | Bacillus subtilis | |
2.5.1.31 | Mn2+ | enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+ | Micrococcus luteus | |
2.5.1.31 | Mn2+ | enzyme is equally stimulated by 0.2 mM Mg2+, 0.2 mM Co2+ or 0.1 mM Mn2+ | Lactiplantibacillus plantarum | |
2.5.1.31 | Mn2+ | best stimulation by Mg2+. Mn2+ and Zn2+ may partially replace Mn2+ | Bacillus subtilis | |
2.5.1.31 | Na+ | stimulates in presence of Mg2+ | Lactiplantibacillus plantarum | |
2.5.1.31 | Na+ | stimulates in presence of Mg2+ | Bacillus subtilis | |
2.5.1.31 | NH4+ | stimulates in presence of Mg2+ | Lactiplantibacillus plantarum | |
2.5.1.31 | NH4+ | stimulates in presence of Mg2+ | Bacillus subtilis | |
2.5.1.31 | Zn2+ | - |
Lactiplantibacillus plantarum | |
2.5.1.31 | Zn2+ | - |
Micrococcus luteus | |
2.5.1.31 | Zn2+ | can partially replace Mg2+ | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.31 | 30000 | - |
2 * 30000, SDS-PAGE | Lactiplantibacillus plantarum |
2.5.1.31 | 52000 | 58000 | sucrose density gradient centrifugation | Lactiplantibacillus plantarum |
2.5.1.31 | 53000 | 60000 | gel filtration | Lactiplantibacillus plantarum |
2.5.1.31 | 56000 | - |
gel filtration, sucrose density gradient centrifugation | Lactiplantibacillus plantarum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.31 | additional information | Lactiplantibacillus plantarum | involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components | ? | - |
? | |
2.5.1.31 | additional information | Bacillus subtilis | involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components | ? | - |
? | |
2.5.1.31 | additional information | Micrococcus luteus | involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.31 | Bacillus subtilis | - |
- |
- |
2.5.1.31 | Lactiplantibacillus plantarum | - |
ATCC 8014 | - |
2.5.1.31 | Micrococcus luteus | - |
- |
- |
2.5.1.31 | Salmonella newington | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.31 | - |
Lactiplantibacillus plantarum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.31 | additional information | - |
- |
Lactiplantibacillus plantarum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.31 | (2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Bacillus subtilis | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
2.5.1.31 | (2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Micrococcus luteus | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
2.5.1.31 | isopentenyl diphosphate + farnesyl diphosphate | - |
Bacillus subtilis | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
2.5.1.31 | isopentenyl diphosphate + farnesyl diphosphate | - |
Salmonella newington | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
2.5.1.31 | isopentenyl diphosphate + farnesyl diphosphate | trans,trans-farnesyl diphosphate and cis,trans-farnesyl diphosphate | Lactiplantibacillus plantarum | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
2.5.1.31 | isopentenyl diphosphate + farnesyl diphosphate | trans,trans-farnesyl diphosphate | Lactiplantibacillus plantarum | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
2.5.1.31 | isopentenyl diphosphate + farnesyl diphosphate | trans,trans-farnesyl diphosphate | Micrococcus luteus | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
2.5.1.31 | isopentenyl diphosphate + geranyl diphosphate | - |
Lactiplantibacillus plantarum | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
2.5.1.31 | isopentenyl diphosphate + geranyl diphosphate | - |
Bacillus subtilis | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
2.5.1.31 | isopentenyl diphosphate + geranyl diphosphate | - |
Micrococcus luteus | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
2.5.1.31 | isopentenyl diphosphate + geranylgeranyl diphosphate | - |
Bacillus subtilis | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
2.5.1.31 | isopentenyl diphosphate + geranylgeranyl diphosphate | - |
Micrococcus luteus | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
2.5.1.31 | isopentenyl diphosphate + geranylgeranyl diphosphate | trans,trans,trans-geranylgeranyl diphosphate | Lactiplantibacillus plantarum | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
2.5.1.31 | isopentenyl diphosphate + geranylneryl diphosphate | cis,trans,trans-geranylneryl diphosphate | Lactiplantibacillus plantarum | ? | - |
? | |
2.5.1.31 | isopentenyl diphosphate + neryl diphosphate | - |
Lactiplantibacillus plantarum | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
2.5.1.31 | isopentenyl diphosphate + neryl diphosphate | - |
Bacillus subtilis | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
2.5.1.31 | isopentenyl diphosphate + neryl diphosphate | - |
Micrococcus luteus | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
2.5.1.31 | additional information | involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components | Lactiplantibacillus plantarum | ? | - |
? | |
2.5.1.31 | additional information | involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components | Bacillus subtilis | ? | - |
? | |
2.5.1.31 | additional information | involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components | Micrococcus luteus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.31 | dimer | 2 * 30000, SDS-PAGE | Lactiplantibacillus plantarum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.31 | 7.5 | - |
- |
Micrococcus luteus |
2.5.1.31 | 7.5 | - |
and a second optimum at pH 10.2, that is 40% of the optimum at pH 7.5 | Lactiplantibacillus plantarum |
2.5.1.31 | 8.5 | - |
- |
Bacillus subtilis |
2.5.1.31 | 10.2 | - |
and a second optimum at pH 7.5 that is higher than the optimum at pH 10.2 | Lactiplantibacillus plantarum |