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Literature summary extracted from

  • Kharel, Y.; Zhang, Y.W.; Fujihashi, M.; Miki, K.; Koyama, T.
    Identification of significant residues for homoallylic substrate binding of Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase (2001), J. Biol. Chem., 276, 28459-28464.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.31 expression of mutant enzymes R197S, R203S and E216Q in Escherichia coli Micrococcus luteus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.31 D221A comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. Km-value for isopentenyl diphosphate within moderate folds to that of the wild-type and slightly decreased enzymatic activity Micrococcus luteus
2.5.1.31 D226A comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. Km-value for isopentenyl diphosphate within moderate folds to that of the wild-type and slightly decreased enzymatic activity Micrococcus luteus
2.5.1.31 E193Q comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. Km-value for isopentenyl diphosphate within moderate folds to that of the wild-type and slightly decreased enzymatic activity Micrococcus luteus
2.5.1.31 E201Q comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. Km-value for isopentenyl diphosphate within moderate folds to that of the wild-type and slightly decreased enzymatic activity Micrococcus luteus
2.5.1.31 E216Q comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. Km-value for isopentenyl diphosphate is about 11.6fold higher than that for the wild-type enzyme, turnover number is about 18fold lower. The major products contain C35 and C50 prenyl chain length Micrococcus luteus
2.5.1.31 E216Q site-directed mutagenesis, Km value increased Micrococcus luteus
2.5.1.31 F73A comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. 32fold increase in Km-value for isopentenyl diphosphate and about 17fold decrease in turnover-number. A mixture of C30 and C50 products is formed Micrococcus luteus
2.5.1.31 F73A yielded shorter chain prenyl diphosphates as their main products Micrococcus luteus
2.5.1.31 R197S comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. Km-value for isopentenyl diphosphate is about 7.2fold higher than that for the wild-type enzyme, turnover-number is 1200fold lower. Compared with the wild-type enzyme lower reaction rate in catalysis and fewer product produced after 6 h reaction Micrococcus luteus
2.5.1.31 R197S site-directed mutagenesis, Km value increased Micrococcus luteus
2.5.1.31 R203S comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. Km-value for isopentenyl diphosphate is about 8fold higher than that for the wild-type enzyme, turnover-number is 1200fold lower. Compared with the wild-type enzyme lower reaction rate in catalysis and fewer product produced after 6 h reaction Micrococcus luteus
2.5.1.31 R203S site-directed mutagenesis, Km value increased Micrococcus luteus
2.5.1.31 S74A comparable Km-values for farnesyl diphosphate and geranylgeranyl diphosphate with those of the wild-type enzyme. 16fold increase in Km-value for isopentenyl diphosphate and about 12fold decrease in turnover-number. The major products contain C35 and C50 prenyl chain length Micrococcus luteus
2.5.1.31 S74A yielded shorter chain prenyl diphosphates as their main products Micrococcus luteus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.31 EDTA
-
 Micrococcus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.31 0.0035
-
 farnesyl diphosphate mutant enzyme D221A, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0053
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme S74A, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0054
-
 farnesyl diphosphate mutant enzyme R216Q, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0058
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme R216Q, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.006
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme R197S, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0066
-
 farnesyl diphosphate mutant enzyme E193Q, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0075
-
 geranylgeranyl diphosphate mutant enzyme D226A, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0078
-
 isopentenyl diphosphate wild-type enzyme, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0081
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme E193Q, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0081
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme R203S, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0082
-
 (Z,E,E)-Geranylgeranyl diphosphate wild-type enzyme, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0083
-
 farnesyl diphosphate wild-type enzyme, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0092
-
 farnesyl diphosphate mutant enzyme F73A, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0094
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme F73A, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0096
-
 farnesyl diphosphate mutant enzyme S74A, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0112
-
 farnesyl diphosphate mutant enzyme D226A, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0116
-
 farnesyl diphosphate mutant enzyme E201Q, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0136
-
 farnesyl diphosphate mutant enzyme R203S, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.017
-
 isopentenyl diphosphate mutant enzyme D4226A, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0177
-
 isopentenyl diphosphate mutant enzyme D221A, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0177
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme E201Q, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.018
-
 farnesyl diphosphate mutant enzyme R197S, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0181
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme D221A, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0183
-
 isopentenyl diphosphate mutant enzyme E201Q, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0204
-
 isopentenyl diphosphate mutant enzyme E193Q, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0561
-
 isopentenyl diphosphate mutant enzyme R197S, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0621
-
 isopentenyl diphosphate mutant enzyme R203S, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.0909
-
 isopentenyl diphosphate mutant enzyme E216Q, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.1268
-
 isopentenyl diphosphate mutant enzyme S74A, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 0.2525
-
 isopentenyl diphosphate mutant enzyme F73A, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 3.5
-
 (2E,6E)-farnesyl diphosphate D221A mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 5.3
-
 (2Z,6E,10E)-geranylgeranyl diphosphate S74A mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 5.4
-
 (2E,6E)-farnesyl diphosphate E216Q mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 5.8
-
 (2Z,6E,10E)-geranylgeranyl diphosphate E216Q mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 6
-
 (2Z,6E,10E)-geranylgeranyl diphosphate R197S mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 6.6
-
 (2E,6E)-farnesyl diphosphate E193Q mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 7.5
-
 (2Z,6E,10E)-geranylgeranyl diphosphate D226A mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 7.8
-
 isopentenyl diphosphate wild type, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 8.1
-
 (2Z,6E,10E)-geranylgeranyl diphosphate E193Q mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 8.1
-
 (2Z,6E,10E)-geranylgeranyl diphosphate R203S mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 8.2
-
 (2Z,6E,10E)-geranylgeranyl diphosphate wild type, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 8.3
-
 (2E,6E)-farnesyl diphosphate wild type, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 9.2
-
 (2E,6E)-farnesyl diphosphate F73A mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 9.4
-
 (2Z,6E,10E)-geranylgeranyl diphosphate F73A mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 9.6
-
 (2E,6E)-farnesyl diphosphate S74A mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 11.2
-
 (2E,6E)-farnesyl diphosphate D226A mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 11.6
-
 (2E,6E)-farnesyl diphosphate E201Q mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 13.6
-
 (2E,6E)-farnesyl diphosphate R203S mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 17
-
 isopentenyl diphosphate D226A mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 17.7
-
 isopentenyl diphosphate D221A mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 17.7
-
 (2Z,6E,10E)-geranylgeranyl diphosphate E201Q mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 18
-
 (2E,6E)-farnesyl diphosphate R197S mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 18.1
-
 (2Z,6E,10E)-geranylgeranyl diphosphate D221A mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 18.3
-
 isopentenyl diphosphate E201Q mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 20.4
-
 isopentenyl diphosphate E193Q mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 56.1
-
 isopentenyl diphosphate R197S mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 62.1
-
 isopentenyl diphosphate R203S mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 90.9
-
 isopentenyl diphosphate E216Q mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 126.8
-
 isopentenyl diphosphate S74A mutant, pH 7.3, 37°C Micrococcus luteus
2.5.1.31 252.5
-
 isopentenyl diphosphate F73A mutant, pH 7.3, 37°C Micrococcus luteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.31 Ca2+ weak stimulation Micrococcus luteus
2.5.1.31 Co2+ weak stimulation Micrococcus luteus
2.5.1.31 Mg2+ divalent metal ion required, Mg2+ is most effective, optimal concentration is 240 nM Micrococcus luteus
2.5.1.31 Mn2+ weak stimulation Micrococcus luteus
2.5.1.31 Zn2+ optimal concentration for activation is about 240 nM Micrococcus luteus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.31 Micrococcus luteus O82827 B-P 26
-
2.5.1.31 Micrococcus luteus B-P 26 O82827 B-P 26
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.31 (2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Micrococcus luteus 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
2.5.1.31 (2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Micrococcus luteus B-P 26 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
2.5.1.31 (2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Micrococcus luteus ?
-
 ?
2.5.1.31 (2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Micrococcus luteus B-P 26 ?
-
 ?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate (Z,E,E)-farnesyl diphosphate Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate E193Q, R197S, E201Q, R203S, D221A, and D226A show product distribution pattern similar to that of the wild-type enzyme, which produces C55 prenyl diphosphate as its major product as well as minor amounts of some intermediates having shorter prenyl chains and C60 product. The major products of the mutant enzymes S74A and E216Q contain C35 and C50 prenyl chain length. Mutant enzyme F73A produces a mixture of C30 and C35 products ?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate (Z,E,E)-farnesyl diphosphate Micrococcus luteus B-P 26 C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate E193Q, R197S, E201Q, R203S, D221A, and D226A show product distribution pattern similar to that of the wild-type enzyme, which produces C55 prenyl diphosphate as its major product as well as minor amounts of some intermediates having shorter prenyl chains and C60 product. The major products of the mutant enzymes S74A and E216Q contain C35 and C50 prenyl chain length. Mutant enzyme F73A produces a mixture of C30 and C35 products ?
2.5.1.31 isopentenyl diphosphate + geranylgeranyl diphosphate (Z,E,E)-geranylgeranyl diphosphate Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + geranylgeranyl diphosphate (Z,E,E)-geranylgeranyl diphosphate Micrococcus luteus B-P 26 C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.31 undecaprenyl diphosphate synthase
-
 Micrococcus luteus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.31 37
-
 assay at Micrococcus luteus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.31 additional information
-
 additional information
-
 Micrococcus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.31 7.3
-
 assay at Micrococcus luteus