EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.84 | bacitracin | 0.03-0.06%, 2fold stimulation | Micrococcus luteus | |
2.5.1.84 | Tween 80 | 0.05-0.1%, 2fold stimulation | Micrococcus luteus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.84 | 0.00025 | - |
all-trans-farnesyl diphosphate | pH 7.4, 37°C | Micrococcus luteus | |
2.5.1.84 | 0.00075 | - |
geranyl diphosphate | pH 7.4, 37°C | Micrococcus luteus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.84 | Mg2+ | Mg2+ is absolutely required for activity, Mn2+ is less effective. The chain length of the synthesized polyprenyl diphosphate is dependent on the concentration of Mg2+ in the medium. At 0.1 mM Mg2+, C25, C30 and C35 compounds are produced with C25 predominating. At 0.5 mM Mg2+, C25, C30, C35 and C40 compounds are produced with C35 and c40 predominating. At 20 mM Mg2+, C40 and C45 are produced without accumulation od shorter compounds | Micrococcus luteus | |
2.5.1.84 | Mn2+ | Mg2+ is absolutely required for activity, Mn2+ is less effective | Micrococcus luteus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.84 | 78000 | - |
gel filtration | Micrococcus luteus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.84 | Micrococcus luteus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.84 | - |
Micrococcus luteus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.84 | 0.000787 | - |
- |
Micrococcus luteus |
EC Number | Storage Stability | Organism |
---|---|---|
2.5.1.84 | -20°C, stable for at least 2 weeks | Micrococcus luteus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.84 | all-trans-farnesyl diphosphate + 6 isopentenyl diphosphate | - |
Micrococcus luteus | 6 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
2.5.1.84 | geranyl diphosphate + 7 isopentenyl diphosphate | - |
Micrococcus luteus | 7 diphosphate + all-trans-nonaprenyl diphosphate | the chain length of the synthesized polyprenyl diphosphate is dependent on the concentration of Mg2+ in the medium. At 0.1 mM Mg2+, C25, C30 and C35 compounds are produced with C25 predominating. At 0.5 mM Mg2+, C25, C30, C35 and C40 compounds are produced with C35 and C40 predominating. At 20 mM Mg2+, C40 and C45 are produced without accumulation od shorter compounds | ? | |
2.5.1.84 | geranylgeranyl diphosphate + 5 isopentenyl diphosphate | - |
Micrococcus luteus | 5 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
2.5.1.84 | additional information | no activity with geranylneryl diphosphate or dimethylallyl diphosphate | Micrococcus luteus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.84 | nonaprenylpyrophosphate synthetase | - |
Micrococcus luteus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.84 | 37 | - |
assay at | Micrococcus luteus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.84 | 7 | 8 | in Tris-HCl buffer | Micrococcus luteus |