EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.7 | AMP | competitive against 5-phospho-alpha-D-ribose 1-diphosphate | Artemia sp. | |
2.4.2.7 | Ba2+ | - |
Artemia sp. | |
2.4.2.7 | Ca2+ | strong | Artemia sp. | |
2.4.2.7 | guanine | noncompetitive against 5-phosphoribose 1-diphosphate | Artemia sp. | |
2.4.2.8 | Ba2+ | - |
Artemia sp. | |
2.4.2.8 | Ca2+ | strong | Artemia sp. | |
2.4.2.8 | GMP | mixed inhibition | Artemia sp. | |
2.4.2.8 | guanine | substrate inhibition | Artemia sp. | |
2.4.2.8 | hypoxanthine | substrate inhibition | Artemia sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.7 | 0.002 | - |
adenine | - |
Artemia sp. | |
2.4.2.7 | 0.03 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Artemia sp. | |
2.4.2.8 | 0.001 | - |
guanine | below | Artemia sp. | |
2.4.2.8 | 0.001 | - |
hypoxanthine | below | Artemia sp. | |
2.4.2.8 | 0.015 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Artemia sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.2.7 | cytosol | - |
Artemia sp. | 5829 | - |
2.4.2.8 | cytosol | - |
Artemia sp. | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.7 | Ca2+ | inhibition | Artemia sp. | |
2.4.2.7 | Mg2+ | required | Artemia sp. | |
2.4.2.7 | Mg2+ | 0.15-0.18 mM | Artemia sp. | |
2.4.2.7 | Mn2+ | Km: 0.04-0.05 | Artemia sp. | |
2.4.2.7 | Mn2+ | activity depends on presence of divalent cations, Mn2+ or Mg2+ activates | Artemia sp. | |
2.4.2.7 | Zn2+ | activates, Km: 0.045-0.05 mM | Artemia sp. | |
2.4.2.8 | Mg2+ | required | Artemia sp. | |
2.4.2.8 | Mg2+ | maximal activity at: 1 mM | Artemia sp. | |
2.4.2.8 | Mg2+ | Km: 0.04-0.05 mM | Artemia sp. | |
2.4.2.8 | Mn2+ | can replace Mg2+ | Artemia sp. | |
2.4.2.8 | Mn2+ | Km: 0.015-0.020 mM | Artemia sp. | |
2.4.2.8 | Zn2+ | activates, can replace Mg2+, Km: 0.066-0.075 mM | Artemia sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.7 | 15000 | - |
2 * 15000, SDS-PAGE | Artemia sp. |
2.4.2.7 | 28000 | - |
gel filtration | Artemia sp. |
2.4.2.8 | 19000 | - |
4 * 19000, SDS-PAGE | Artemia sp. |
2.4.2.8 | 66000 | - |
gel filtration | Artemia sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.7 | Artemia sp. | - |
- |
- |
2.4.2.8 | Artemia sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.7 | - |
Artemia sp. |
2.4.2.8 | - |
Artemia sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.2.7 | cyst | - |
Artemia sp. | - |
2.4.2.7 | nauplius | - |
Artemia sp. | - |
2.4.2.8 | cyst | - |
Artemia sp. | - |
2.4.2.8 | nauplius | - |
Artemia sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.2.7 | 14.2 | - |
purified enzyme | Artemia sp. |
2.4.2.8 | 0.65 | - |
purified enzyme | Artemia sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.7 | adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Artemia sp. | AMP + diphosphate | - |
? | |
2.4.2.8 | guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Artemia sp. | GMP + diphosphate | - |
? | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Artemia sp. | IMP + diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.7 | dimer | 2 * 15000, SDS-PAGE | Artemia sp. |
2.4.2.8 | tetramer | 4 * 19000, SDS-PAGE | Artemia sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.7 | 37 | - |
assay at | Artemia sp. |
2.4.2.8 | 37 | - |
assay at | Artemia sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.7 | 8 | 9 | - |
Artemia sp. |
2.4.2.8 | 7 | - |
2 buffer-independent pH-optima: pH 7.0 and pH 9.5 | Artemia sp. |
2.4.2.8 | 9.5 | - |
2 buffer-independent pH-optima: pH 7.0 and pH 9.5 | Artemia sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.8 | 0.01 | - |
guanine | - |
Artemia sp. | |
2.4.2.8 | 0.08 | - |
hypoxanthine | - |
Artemia sp. |