Literature summary extracted from
Thrower, J.S.; Blalock, R.; Klinman, J.P.
Steady-state kinetics of substrate binding and iron release in tomato ACC oxidase (2001), Biochemistry, 40, 9717-9724.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.14.17.4 |
ascorbate |
required |
Solanum lycopersicum |
|
1.14.17.4 |
CO2 |
activates |
Solanum lycopersicum |
|
1.14.17.4 |
additional information |
no activation by bisulfite, isozyme AOC1 |
Solanum lycopersicum |
|
1.14.17.4 |
O2 |
required |
Solanum lycopersicum |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.17.4 |
isozyme ACO1, overexpression in Escherichia coli BL21(DE3)-pLysS |
Solanum lycopersicum |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.14.17.4 |
1-aminocyclopropane-1-carboxylate |
slight inhibition at high concentration |
Solanum lycopersicum |
|
1.14.17.4 |
Zn2+ |
competitive to Fe2+ |
Solanum lycopersicum |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
1.14.17.4 |
0.028 |
- |
O2 |
recombinant isozyme ACO1, in presence of 0.5 mM L-ascorbate and 20 mM CO2/HCO3-, pH 7.2, 25°C |
Solanum lycopersicum |
|
1.14.17.4 |
0.077 |
- |
1-aminocyclopropane-1-carboxylate |
recombinant isozyme ACO1, in presence of 0.5 mM L-ascorbate and 20 mM CO2/HCO3-, pH 7.2, 25°C |
Solanum lycopersicum |
|
1.14.17.4 |
4.6 |
- |
L-ascorbate |
recombinant isozyme ACO1, in presence of 0.5 mM L-ascorbate and 20 mM CO2/HCO3-, pH 7.2, 25°C |
Solanum lycopersicum |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.14.17.4 |
Fe2+ |
required |
Solanum lycopersicum |
|
1.14.17.4 |
Fe2+ |
a single bound Fe2+ per monomer |
Solanum lycopersicum |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.14.17.4 |
1-aminocyclopropane-1-carboxylate + ascorbate + O2 |
Solanum lycopersicum |
catalyses the final step in ethylene biosynthesis |
ethylene + cyanide + dehydroascorbate + CO2 + H2O |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.17.4 |
Solanum lycopersicum |
- |
isozyme ACO1 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.17.4 |
recombinant isozyme ACO1 from Escherichia coli BL21(DE3)-pLysS, to near homogeneity |
Solanum lycopersicum |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
1.14.17.4 |
1-aminocyclopropane-1-carboxylate + ascorbate + O2 = ethene + cyanide + dehydroascorbate + CO2 + 2 H2O |
catalytic mechanism |
Solanum lycopersicum |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.17.4 |
1-aminocyclopropane-1-carboxylate + ascorbate + O2 |
catalyses the final step in ethylene biosynthesis |
Solanum lycopersicum |
ethylene + cyanide + dehydroascorbate + CO2 + H2O |
- |
? |
|
1.14.17.4 |
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 |
specific for the substrates |
Solanum lycopersicum |
ethylene + cyanide + dehydroascorbate + CO2 + H2O |
- |
? |
|
1.14.17.4 |
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 |
i.e. ACC |
Solanum lycopersicum |
ethylene + cyanide + dehydroascorbate + CO2 + H2O |
- |
? |
|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.14.17.4 |
25 |
- |
assay at |
Solanum lycopersicum |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.14.17.4 |
7.2 |
- |
assay at |
Solanum lycopersicum |
Ki Value [mM]
EC Number |
Ki Value [mM] |
Ki Value maximum [mM] |
Inhibitor |
Comment |
Organism |
Structure |
---|
1.14.17.4 |
38.9 |
- |
1-aminocyclopropane-1-carboxylate |
recombinant isozyme ACO1, in presence of 5 mM L-ascorbate and 20 mM CO2/HCO3-, pH 7.2, 25°C |
Solanum lycopersicum |
|