Literature summary extracted from

Cooper, A.J.L.; Meister, A.
Glutamine transaminase L from rat liver (1985), Methods Enzymol., 113, 338-343.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.6.1.15	Aminoxyacetate	soluble fraction	Rattus norvegicus
2.6.1.15	cycloserine	soluble fraction	Rattus norvegicus
2.6.1.15	hydroxylamine	soluble fraction	Rattus norvegicus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.6.1.15	cytosol	-	Rattus norvegicus	5829	-
2.6.1.15	mitochondrion	-	Rattus norvegicus	5739	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.6.1.15	110000	-	-	Rattus norvegicus

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.6.1.15	Rattus norvegicus	-	male Sprague-Dawley	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.6.1.15	DE-52 chromatography, hydroxyapatite chromatography for soluble fraction, DE-52 chromatography for mitochondrial fraction	Rattus norvegicus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.6.1.15	liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.6.1.15	0.113	-	mitochondrion	Rattus norvegicus
2.6.1.15	5	-	soluble fraction	Rattus norvegicus

Storage Stability

EC Number	Storage Stability	Organism
2.6.1.15	4ºC, 10 mM potassium phosphate, pH 7.2, 10% glycerol, 1 mM 2-mercaptoethanol, 0.01 mM pyridoxal 5'-phosphate, 3 months	Rattus norvegicus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.6.1.15	glyoxylate + L-glutamine	best substrate	Rattus norvegicus	glycine + 2-oxoglutaramate	-	?
2.6.1.15	glyoxylate + L-methionine	-	Rattus norvegicus	glycine + 4-methylsulfanyl-2-oxobutanoate	-	?
2.6.1.15	L-glutamine + 2-oxoglutaramate	-	Rattus norvegicus	2-oxoglutaramate + L-glutamine	-	?
2.6.1.15	L-glutamine + 3-mercaptopyruvate	-	Rattus norvegicus	2-oxoglutaramate + L-cysteine	-	?

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.6.1.15	37	-	assay at	Rattus norvegicus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)