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Literature summary extracted from

  • Ghosh, S.; Blumenthal, H.J.; Davidson, E.; Roseman, S.
    Glucosamine metabolism.V. Enzymatic synthesis of glucosamine 6-phosphate (1960), J. Biol. Chem., 235, 1265-1273.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.16 inactivated by freezing and thawing Escherichia coli
2.6.1.16 inactivated when exposed to a solution of pH 5.5 or below Escherichia coli
2.6.1.16 inactivated when exposed to a solution of pH 5.5 or below Rattus norvegicus
2.6.1.16 inactivated when exposed to a solution of pH 5.5 or below Neurospora crassa
2.6.1.16 very unstable Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.16 6-diazo-5-oxo-L-norleucine
-
 Escherichia coli
2.6.1.16 6-diazo-5-oxo-L-norleucine
-
 Neurospora crassa
2.6.1.16 6-diazo-5-oxo-L-norleucine
-
 Rattus norvegicus
2.6.1.16 azaserine weak Escherichia coli
2.6.1.16 azaserine
-
 Neurospora crassa
2.6.1.16 azaserine weak Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.16 0.38
-
 D-fructose 6-phosphate pH 7.7, 37°C Rattus norvegicus
2.6.1.16 0.65
-
 L-glutamine pH 7.0, 37°C Escherichia coli
2.6.1.16 0.75
-
 D-fructose 6-phosphate pH 7.0, 37°C Neurospora crassa
2.6.1.16 1.6
-
 L-glutamine pH 7.7, 37°C Rattus norvegicus
2.6.1.16 2
-
 D-fructose 6-phosphate pH 7.0, 37°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.16 Aspergillus flavus
-
-
-
2.6.1.16 Aspergillus parasiticus
-
-
-
2.6.1.16 Colletotrichum gloeosporioides
-
-
-
2.6.1.16 Escherichia coli
-
-
-
2.6.1.16 Helminthosporium sativum
-
-
-
2.6.1.16 Hypomyces rosellus
-
-
-
2.6.1.16 Neurospora crassa
-
-
-
2.6.1.16 Neurospora tetrasperma
-
-
-
2.6.1.16 Penicillium chrysogenum
-
-
-
2.6.1.16 Penicillium sp.
-
-
-
2.6.1.16 Rattus norvegicus
-
 rat
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.16 liver
-
 Rattus norvegicus
-
2.6.1.16 liver
-
 Penicillium chrysogenum
-
2.6.1.16 liver
-
 Aspergillus flavus
-
2.6.1.16 liver
-
 Colletotrichum gloeosporioides
-
2.6.1.16 liver
-
 Aspergillus parasiticus
-
2.6.1.16 liver
-
 Penicillium sp.
-
2.6.1.16 liver
-
 Hypomyces rosellus
-
2.6.1.16 liver
-
 Helminthosporium sativum
-
2.6.1.16 liver
-
 Neurospora tetrasperma
-

Storage Stability

EC Number Storage Stability Organism
2.6.1.16 -18°C, little loss of activity after 1 week for lyophilized material after calcium phosphate gel treatment Neurospora crassa
2.6.1.16 0-2°C, potassium phosphate buffer, pH 7.0, 0.06 M L-glutamine, 0.01 M EDTA, stable for up to 3 or 4 weeks Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Penicillium chrysogenum L-glutamate + D-glucosamine 6-phosphate
-
 ?
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Aspergillus flavus L-glutamate + D-glucosamine 6-phosphate
-
 ?
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Colletotrichum gloeosporioides L-glutamate + D-glucosamine 6-phosphate
-
 ?
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Aspergillus parasiticus L-glutamate + D-glucosamine 6-phosphate
-
 ?
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Penicillium sp. L-glutamate + D-glucosamine 6-phosphate
-
 ?
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Hypomyces rosellus L-glutamate + D-glucosamine 6-phosphate
-
 ?
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Helminthosporium sativum L-glutamate + D-glucosamine 6-phosphate
-
 ?
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Neurospora tetrasperma L-glutamate + D-glucosamine 6-phosphate
-
 ?
2.6.1.16 L-glutamine + D-fructose 6-phosphate specific for: L-glutamine Escherichia coli L-glutamate + D-glucosamine 6-phosphate
-
 ir
2.6.1.16 L-glutamine + D-fructose 6-phosphate specific for: L-glutamine Rattus norvegicus L-glutamate + D-glucosamine 6-phosphate
-
 ir
2.6.1.16 L-glutamine + D-fructose 6-phosphate specific for: L-glutamine Neurospora crassa L-glutamate + D-glucosamine 6-phosphate
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.16 37
-
 assay at Escherichia coli
2.6.1.16 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.16 6.7
-
-
 Neurospora crassa
2.6.1.16 7.7
-
 assay at Escherichia coli
2.6.1.16 7.7
-
 assay at Rattus norvegicus
2.6.1.16 7.7 7.9
-
 Rattus norvegicus
2.6.1.16 7.9
-
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.16 additional information no cofactor requirement Escherichia coli
2.6.1.16 additional information no cofactor requirement Rattus norvegicus
2.6.1.16 additional information no cofactor requirement Neurospora crassa