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Literature summary extracted from

  • Espinal, J.; Beggs, M.; Randle, P.J.
    Assay of branched-chain alpha-keto acid dehydrogenase kinase in mitochondrial extracts and purified branched-chain alpha-keto acid dehydrogenase complexes (1988), Methods Enzymol., 166, 166-175.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.11.4 precipitation of branched-chain oxo acid dehydrogenase enzyme complex at acid pH-values, especially below 6.5, results in specific loss of kinase activity Rattus norvegicus
2.7.11.4 precipitation of branched-chain oxo acid dehydrogenase enzyme complex at acid pH-values, especially below 6.5, results in specific loss of kinase activity Bos taurus
2.7.11.4 precipitation of branched-chain oxo acid dehydrogenase enzyme complex at acid pH-values, especially below 6.5, results in specific loss of kinase activity Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.4 2-(N-Morpholino)propane sulfonate buffer
-
 Bos taurus
2.7.11.4 2-(N-Morpholino)propane sulfonate buffer
-
 Oryctolagus cuniculus
2.7.11.4 2-(N-Morpholino)propane sulfonate buffer
-
 Rattus norvegicus
2.7.11.4 2-oxohexanedioate
-
 Bos taurus
2.7.11.4 2-oxohexanedioate
-
 Oryctolagus cuniculus
2.7.11.4 2-oxohexanedioate
-
 Rattus norvegicus
2.7.11.4 2-oxopentanoate
-
 Bos taurus
2.7.11.4 2-oxopentanoate
-
 Oryctolagus cuniculus
2.7.11.4 acetoacetyl-CoA
-
 Bos taurus
2.7.11.4 acetoacetyl-CoA
-
 Oryctolagus cuniculus
2.7.11.4 acetoacetyl-CoA
-
 Rattus norvegicus
2.7.11.4 ADP
-
 Bos taurus
2.7.11.4 ADP
-
 Oryctolagus cuniculus
2.7.11.4 ADP
-
 Rattus norvegicus
2.7.11.4 methylmalonyl-CoA
-
 Bos taurus
2.7.11.4 methylmalonyl-CoA
-
 Oryctolagus cuniculus
2.7.11.4 methylmalonyl-CoA
-
 Rattus norvegicus
2.7.11.4 n-Octanoate
-
 Bos taurus
2.7.11.4 n-Octanoate
-
 Oryctolagus cuniculus
2.7.11.4 n-Octanoate
-
 Rattus norvegicus
2.7.11.4 thiamine diphosphate
-
 Bos taurus
2.7.11.4 thiamine diphosphate
-
 Oryctolagus cuniculus
2.7.11.4 thiamine diphosphate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.4 0.013
-
 ATP pH 7.5, 30°C Bos taurus
2.7.11.4 0.013
-
 ATP ATP in form of MgATP2- Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.4 mitochondrion
-
 Rattus norvegicus 5739
-
2.7.11.4 mitochondrion
-
 Bos taurus 5739
-
2.7.11.4 mitochondrion
-
 Oryctolagus cuniculus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.4 Mg2+ requirement, actual substrate: MgATP2- Rattus norvegicus
2.7.11.4 Mg2+ requirement, actual substrate: MgATP2- Bos taurus
2.7.11.4 Mg2+ requirement, actual substrate: MgATP2- Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] Rattus norvegicus phosphorylation inactivates EC 1.2.4.4 ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] Bos taurus phosphorylation inactivates EC 1.2.4.4 ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] Oryctolagus cuniculus phosphorylation inactivates EC 1.2.4.4 ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.4 Bos taurus
-
-
-
2.7.11.4 Oryctolagus cuniculus
-
-
-
2.7.11.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.4 from liver and heart, homogeneity Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.4 adipocyte
-
 Rattus norvegicus
-
2.7.11.4 heart
-
 Rattus norvegicus
-
2.7.11.4 kidney
-
 Rattus norvegicus
-
2.7.11.4 kidney
-
 Bos taurus
-
2.7.11.4 kidney
-
 Oryctolagus cuniculus
-
2.7.11.4 liver
-
 Rattus norvegicus
-
2.7.11.4 liver
-
 Bos taurus
-
2.7.11.4 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.4 additional information
-
 various assay methods Rattus norvegicus
2.7.11.4 additional information
-
 various assay methods Bos taurus
2.7.11.4 additional information
-
 various assay methods Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)]
-
 Bos taurus ADP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] phosphate
-
 ?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] 2 Ser-residues in E1-alpha-subunit Rattus norvegicus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] 2 Ser-residues in E1-alpha-subunit Bos taurus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] 2 Ser-residues in E1-alpha-subunit Oryctolagus cuniculus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphorylation inactivates EC 1.2.4.4 Rattus norvegicus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphorylation inactivates EC 1.2.4.4 Bos taurus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphorylation inactivates EC 1.2.4.4 Oryctolagus cuniculus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.11.4 0.27
-
 ADP pH 7.5, 30°C Bos taurus