Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • McMorrow, E.M.; Bradbeer, J.W.
    Separation, purification, and comparative properties of chloroplast and cytoplasmic phosphoglycerate kinase from barley leaves (1990), Plant Physiol., 93, 374-383.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.2.3 chloroplast 90% of total enzyme activity Hordeum vulgare 9507
-
2.7.2.3 cytosol 10% of total enzyme activity Hordeum vulgare 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 38000
-
 cytoplasmic and chloroplastic isoenzyme, gel filtration Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Hordeum vulgare
-
 2 isozymes, cytosolic and chloroplastic
-
2.7.2.3 Hordeum vulgare
-
 cv. Golden Promise
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3 14 days old leaves, 545fold purification of the chloroplastic and 530fold of the cytoplasmic isoenzyme Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.3 leaf
-
 Hordeum vulgare
-
2.7.2.3 seedling
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.3 69
-
 purified cytosolic isozyme Hordeum vulgare
2.7.2.3 644
-
 purified chloroplastic isozyme Hordeum vulgare
2.7.2.3 914
-
 purified isozymes Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Hordeum vulgare ADP + 1,3-diphosphoglycerate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.3 monomer 1 * 38000, chloroplastic and cytoplasmic enzyme Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.3 30
-
 assay at Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.3 7 8
-
 Hordeum vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Hordeum vulgare
2.7.2.3 ATP required as phosphate donor Hordeum vulgare

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.2.3 Hordeum vulgare chloroplastic isozyme, isoelectric focusing
-
 5.2
2.7.2.3 Hordeum vulgare cytsolic isozyme, isoelectric focusing
-
 5.4