EC Number | General Stability | Organism |
---|---|---|
1.1.99.29 | repeated freezing and thawing, two cycles, in the absence of glycerol has no effect on PDH activity | Chlorophyllum rhacodes |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.29 | 1.82 | - |
D-glucose | 25°C, ferricenium ion as electron acceptor | Chlorophyllum rhacodes | |
1.1.99.29 | 24 | - |
L-arabinose | 25°C, ferricenium ion as electron acceptor | Chlorophyllum rhacodes | |
1.1.99.29 | 29.2 | - |
D-xylose | 25°C, ferricenium ion as electron acceptor | Chlorophyllum rhacodes | |
1.1.99.29 | 29.8 | - |
D-galactose | 25°C, ferricenium ion as electron acceptor | Chlorophyllum rhacodes |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.99.29 | extracellular | active extracellular secretion of PDH | Chlorophyllum rhacodes | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.99.29 | 76000 | - |
gel filtration | Chlorophyllum rhacodes |
1.1.99.29 | 78000 | - |
1 * 78000, SDS-PAGE | Chlorophyllum rhacodes |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.29 | Chlorophyllum rhacodes | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.1.99.29 | glycoprotein | heavily glycosylated, the sugar moiety of PDH comprises 17.3% of its total molecular mass | Chlorophyllum rhacodes |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.29 | 46fold | Chlorophyllum rhacodes |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.99.29 | mycelium | - |
Chlorophyllum rhacodes | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.99.29 | 67 | - |
25°C, D-glucose and ferricenium ion as substrates | Chlorophyllum rhacodes |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.99.29 | 4°C, purified PDH, 20 mM sodium phosphate buffer, pH 7, 0.02% ProClin 300, 2 months, no loss of activity | Chlorophyllum rhacodes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.29 | 2-deoxy-D-glucose + 1,4-benzoquinone | 49% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | 2-deoxy-D-glucose + ferricenium ion | 21% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | 3-dehydro-D-glucose + 1,4-benzoquinone | - |
Chlorophyllum rhacodes | 2,3-didehydro-D-glucose + hydroquinone | - |
? | |
1.1.99.29 | a pyranoside + acceptor | - |
Chlorophyllum rhacodes | a 3-dehydropyranoside (or 3,4-didehydropyranoside) + reduced acceptor | - |
? | |
1.1.99.29 | cellobiose + 1,4-benzoquinone | 13% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | cellobiose + ferricenium ion | 7% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | D-galactose + 1,4-benzoquinone | 66% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | D-galactose + ferricenium ion | 60% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | D-glucono-1,5-lactone + 1,4-benzoquinone | 20% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | D-glucono-1,5-lactone + ferricenium ion | 8% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | D-glucose + 1,4-benzoquinone | oxidizes D-glucose exclusively at C-3, D-glucose is the preferred electron donor susbtrate | Chlorophyllum rhacodes | 3-dehydro-D-glucose + hydroquinone | - |
? | |
1.1.99.29 | D-glucose + ferricenium ion | oxidizes D-glucose exclusively at C-3, D-glucose is the preferred electron donor susbtrate | Chlorophyllum rhacodes | 3-dehydro-D-glucose + ferrocene | - |
? | |
1.1.99.29 | D-lyxose + 1,4-benzoquinone | 5% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | D-lyxose + ferricenium ion | 5% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | D-mannose + 1,4-benzoquinone | 32% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | D-mannose + ferricenium ion | 26% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | D-xylose + 1,4-benzoquinone | 72% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | D-xylose + ferricenium ion | 81% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | L-arabinose + 1,4-benzoquinone | 54% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | L-arabinose + ferricenium ion | 72% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | L-glucose + ferricenium ion | 11% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | maltose + 1,4-benzoquinone | 64% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | maltose + ferricenium ion | 45% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | maltotriose + 1,4-benzoquinone | 21% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | maltotriose + ferricenium ion | 14% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | methyl-alpha-D-galactopyranoside + 1,4-benzoquinone | 11% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | methyl-alpha-D-galactopyranoside + ferricenium ion | 8% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | methyl-alpha-D-glucopyranoside + 1,4-benzoquinone | 141% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | methyl-alpha-D-glucopyranoside + ferricenium ion | 143% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | methyl-beta-D-glucopyranoside + 1,4-benzoquinone | 30% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | methyl-beta-D-glucopyranoside + ferricenium ion | 17% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | additional information | broad selectivity for sugar substrates, O2 does not act as oxidant, not: D-allose, D-altrose, D-arabinose, D-ribose, D-fructose, L-sorbose, lactose, D-arabinitol, dulcitol, D-mannitol | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | pyranose + acceptor | - |
Chlorophyllum rhacodes | 2-dehydropyranose (or 3-dehydropyranose or 2,3-didehydropyranose) + reduced acceptor | - |
? | |
1.1.99.29 | sucrose + 1,4-benzoquinone | 9% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | sucrose + ferricenium ion | 6% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | trehalose + 1,4-benzoquinone | 7% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
1.1.99.29 | trehalose + ferricenium ion | 8% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.99.29 | monomer | 1 * 78000, SDS-PAGE | Chlorophyllum rhacodes |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.29 | 65 | - |
ferricenium ion as electron acceptor | Chlorophyllum rhacodes |
1.1.99.29 | 75 | - |
1,4-benzoquinone as electron acceptor | Chlorophyllum rhacodes |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.29 | 3.5 | - |
50 mM sodium citrate buffer, 1,4-benzoquinone as electron acceptor | Chlorophyllum rhacodes |
1.1.99.29 | 9.5 | - |
50 mM glycine buffer, ferricenium ion as electron acceptor | Chlorophyllum rhacodes |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.29 | FAD | functions as the cofactor | Chlorophyllum rhacodes | |
1.1.99.29 | flavin | flavoprotein, FAD as cofactor | Chlorophyllum rhacodes |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.1.99.29 | Chlorophyllum rhacodes | isoelectric focusing | - |
4.2 |