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Literature summary extracted from

  • McManaman, J.L.; Neville, M.C.; Wright, R.M.
    Mouse mammary gland xanthine oxidoreductase: Purification, characterization, and regulation (1999), Arch. Biochem. Biophys., 371, 308-316.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.1.4 thiol active compounds conversion of oxidase to dehydrogenase form Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.3.2 lipid droplet milk lipid globule membrane possesses enzyme activity capable of catalyzing the conversion of xanthine dehydrogenase to xanthine oxidase, kinetic of this process is consistent with the rapid appearence of xanthine oxidase in milk Mus musculus 5811
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.3.2 Iron iron-molybdenum protein Mus musculus
1.17.3.2 Molybdenum an iron-molybdenum protein Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.4 150000
-
 x * 150000, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 xanthine + NAD+ + H2O Mus musculus xanthine oxidase form is the principle major form in fresh mouse milk, dehydrogenase form is the major form in mammary gland, conversion to the dehydrogenase form by thiol active compounds urate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Mus musculus
-
-
-
1.17.3.2 Mus musculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.17.3.2 additional information
-
 Mus musculus
1.17.3.2 proteolytic modification
-
 Mus musculus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4 to homogeneity, affinity chromatography Mus musculus
1.17.3.2 60% ammonium sulfate, benzamidine-Sepharose Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 mammary gland
-
 Mus musculus
-
1.17.1.4 milk
-
 Mus musculus
-
1.17.3.2 mammary gland
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.3.2 5.2
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 xanthine + 2,6-dichlorophenolindophenol + H2O
-
 Mus musculus urate + ?
-
 ?
1.17.1.4 xanthine + NAD+ + H2O xanthine oxidase form is the principle major form in fresh mouse milk, dehydrogenase form is the major form in mammary gland, conversion to the dehydrogenase form by thiol active compounds Mus musculus urate + NADH
-
 ?
1.17.3.2 hypoxanthine + 2 H2O + 2 O2
-
 Mus musculus urate + 2 H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2 electron acceptor O2 Mus musculus uric acid + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.4 ? x * 150000, SDS-PAGE Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 NAD+
-
 Mus musculus
1.17.3.2 FAD flavoprotein Mus musculus