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Literature summary extracted from

  • Reuter, K.; Chong, S.; Ullrich, F.; Kersten, H.; Garcia, G.A.
    Serine 90 is required for enzymic activity by tRNA-guanine transglycosylase from Escherichia coli (1994), Biochemistry, 33, 7041-7046.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.29 mutant enzymes S90F and S90C Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.29 S90A activity of the mutant enzyme is to low to determine Vmax and Km-value Escherichia coli
2.4.2.29 S90C 30fold increase in Km-value for tRNATyr and 4fold increase in Km-value for guanine Escherichia coli
2.4.2.29 S90F mutant enzyme has no detectable solubility and reduced solubility Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.29 0.00032
-
 tRNA(Tyr) wild-type enzyme Escherichia coli
2.4.2.29 0.0011
-
 guanine mutant enzyme S90A Escherichia coli
2.4.2.29 0.003
-
 guanine wild-type enzyme Escherichia coli
2.4.2.29 0.0097
-
 tRNA(Tyr) mutant enzyme S90C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.29 guanine34 in tRNA + 7-aminomethyl-7-carbaguanine Escherichia coli the enzyme catalyzes the posttranscriptional base exchange of the queuosine precursor 7-(aminomethyl)-7-deazaguanine with the genetically encoded guanine in tRNAASp, tRNAAsn, tRNAHis and tRNATyr 7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.29 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.29 mutant enzyme S90F, S90C and S90A Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.29 guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
-
 Escherichia coli 7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
 ?
2.4.2.29 guanine34 in tRNA + 7-aminomethyl-7-carbaguanine the enzyme catalyzes the posttranscriptional base exchange of the queuosine precursor 7-(aminomethyl)-7-deazaguanine with the genetically encoded guanine in tRNAASp, tRNAAsn, tRNAHis and tRNATyr Escherichia coli 7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
 ?