Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ruijssenaars, H.J.; de Bont, J.A.; Hartmans, S.
    A pyruvated mannose-specific xanthan lyase involved in xanthan degradation by Paenibacillus alginolyticus XL-1 (1999), Appl. Environ. Microbiol., 65, 2446-2452.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.12 CuCl2 0.1 mM, 70% loss of activity Paenibacillus alginolyticus
4.2.2.12 EDTA 10 mM, not Paenibacillus alginolyticus
4.2.2.12 HgCl2 0.1 mM, complete inhibition Paenibacillus alginolyticus
4.2.2.12 additional information not inhibitory: CoCl2, MgCl2, CaCl2 at 1 mM Paenibacillus alginolyticus
4.2.2.12 NaCl below 10 mM, not, at 85 mM, 60% loss of activity Paenibacillus alginolyticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.12 soluble excreted to medium Paenibacillus alginolyticus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.12 85000
-
 x * 85000, SDS-PAGE Paenibacillus alginolyticus

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.12 Paenibacillus alginolyticus
-
 enzyme is induced by xanthan
-
4.2.2.12 Paenibacillus alginolyticus XL-1
-
 enzyme is induced by xanthan
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.12
-
 Paenibacillus alginolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.12 2.62
-
 30°C, pH 6.9 Paenibacillus alginolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.12 xanthan
-
 Paenibacillus alginolyticus oligosaccharides
-
 ?
4.2.2.12 xanthan
-
 Paenibacillus alginolyticus XL-1 oligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.12 ? x * 85000, SDS-PAGE Paenibacillus alginolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.12 55
-
-
 Paenibacillus alginolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.12 45
-
 not stable above Paenibacillus alginolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.12 6
-
-
 Paenibacillus alginolyticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.12 Paenibacillus alginolyticus isoelectric focusing
-
 7.9