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Literature summary extracted from

  • Liu, S.; Lu, Z.; Jia, Y.; Dunaway-Mariano, D.; Herzberg, O.
    Dissociative phosphoryl transfer in PEP mutase catalysis: structure of the enzyme/sulfopyruvate complex and kinetic properties of mutants (2002), Biochemistry, 41, 10270-10276.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.2.9
-
 Mytilus edulis

Protein Variants

EC Number Protein Variants Comment Organism
5.4.2.9 D58A no enzymic activity Mytilus edulis
5.4.2.9 D58N Km: 0.0078 mM Mytilus edulis
5.4.2.9 D58S no enzymic activity Mytilus edulis
5.4.2.9 H190A Km: 0.0065 mM Mytilus edulis
5.4.2.9 N122A Km: 0.038 mM Mytilus edulis
5.4.2.9 N122D Km: 0.0032 mM Mytilus edulis
5.4.2.9 R159A Km: 0.054 mM Mytilus edulis

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.2.9 sulfopyruvate
-
 Mytilus edulis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.9 0.0016
-
 Phosphonopyruvate 25°C, pH 7.5 Mytilus edulis
5.4.2.9 0.0032
-
 Phosphonopyruvate mutant N122D, 25°C, pH 7.5 Mytilus edulis
5.4.2.9 0.0065
-
 Phosphonopyruvate mutant H190A, 25°C, pH 7.5 Mytilus edulis
5.4.2.9 0.0078
-
 Phosphonopyruvate mutant D58N, 25°C, pH 7.5 Mytilus edulis
5.4.2.9 0.038
-
 Phosphonopyruvate mutant N122A, 25°C, pH 7.5 Mytilus edulis
5.4.2.9 0.054
-
 Phosphonopyruvate mutant R159A, 25°C, pH 7.5 Mytilus edulis

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.9 Mytilus edulis P56839
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
5.4.2.9 phosphoenolpyruvate = 3-phosphonopyruvate mechanism Mytilus edulis
a

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.4.2.9 0.022
-
 sulfopyruvate 25°C, pH 7.5 Mytilus edulis