Literature summary extracted from
Sehgal, A.C.; Tompson, R.; Cavanagh, J.; Kelly, R.M.
Structural and catalytic response to temperature and cosolvents of carboxylesterase EST1 from the extremely thermoacidophilic archaeon Sulfolobus solfataricus P1 (2002), Biotechnol. Bioeng., 80, 784-793.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.1.1.1 |
DMSO |
optimal activation at 3.5% v/v with an increase in kcat of 20-30%, no dramatic changes in conformation of the enzyme at 3.5% v/v DMSO and 50-80°C, but significant changes at small DMSO concentrations |
Saccharolobus solfataricus |
|
Application
EC Number |
Application |
Comment |
Organism |
---|
3.1.1.1 |
biotechnology |
the enzyme can be used as a biocatalyst at suboptimal temperatures through addition of DMSO as an activator |
Saccharolobus solfataricus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.1.1 |
acetonitrile |
inactivation |
Saccharolobus solfataricus |
|
3.1.1.1 |
Dimethyl formamide |
inactivation |
Saccharolobus solfataricus |
|
3.1.1.1 |
dioxane |
inactivation |
Saccharolobus solfataricus |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.1.1.1 |
additional information |
- |
additional information |
kinetics |
Saccharolobus solfataricus |
|
Organic Solvent Stability
EC Number |
Organic Solvent |
Comment |
Organism |
---|
3.1.1.1 |
acetonitrile |
inactivation |
Saccharolobus solfataricus |
3.1.1.1 |
dimethyl formamide |
inactivation |
Saccharolobus solfataricus |
3.1.1.1 |
dioxane |
inactivation |
Saccharolobus solfataricus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.1.1 |
Saccharolobus solfataricus |
- |
enzyme Est1 |
- |
3.1.1.1 |
Saccharolobus solfataricus P1 |
- |
enzyme Est1 |
- |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.1.1 |
Est1 |
- |
Saccharolobus solfataricus |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.1.1.1 |
50 |
80 |
- |
Saccharolobus solfataricus |