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Literature summary extracted from

  • Honda, K.; Kataoka, M.; Sakuradani, E.; Shimizu, S.
    Role of Acinetobacter calcoaceticus 3,4-dihydrocoumarin hydrolase in oxidative stress defence against peroxoacids (2003), Eur. J. Biochem., 270, 486-494.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.35 expression in Escherichia coli Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.35 0.39
-
 peracetic acid 30°C, pH 5.0 Acinetobacter calcoaceticus
3.1.1.35 0.8
-
 3,4-dihydrocoumarin 30°C, pH 7.0 Acinetobacter calcoaceticus
3.1.1.35 25.9
-
 DL-beta-acetylthioisobutyrate methyl ester 30°C, pH 7.0 Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.35 dihydrocoumarin + H2O Acinetobacter calcoaceticus
-
 melilotate
-
 ?
3.1.1.35 dihydrocoumarin + H2O Acinetobacter calcoaceticus F46
-
 melilotate
-
 ?
3.1.1.35 peracetic acid Acinetobacter calcoaceticus peracetic acid is likely a natural substrate in vivo, enzyme may play a role in oxidative stress defense system acetic acid + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.35 Acinetobacter calcoaceticus Q83WC8
-
-
3.1.1.35 Acinetobacter calcoaceticus F46 Q83WC8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.35
-
 Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.35 14.3
-
 monochlordimedonbrominating activity, recombinant enzyme Acinetobacter calcoaceticus
3.1.1.35 116
-
 3,4-dihydrocoumarin hydrolysis, recombinant enzyme Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.35 2-chloro-5,5-dimethyl-1,3-cyclohexanedimedon + Br- + peracetic acid
-
 Acinetobacter calcoaceticus 2-chloro-2-bromo-5,5-dimethyl-1,3-cyclohexanedimedon + acetic acid + H2O
-
 ?
3.1.1.35 2-chloro-5,5-dimethyl-1,3-cyclohexanedimedon + Br- + peracetic acid
-
 Acinetobacter calcoaceticus F46 2-chloro-2-bromo-5,5-dimethyl-1,3-cyclohexanedimedon + acetic acid + H2O
-
 ?
3.1.1.35 3,4-dihydrocoumarin + H2O
-
 Acinetobacter calcoaceticus 3-(2-hydroxyphenyl)propionic acid
-
 ?
3.1.1.35 3,4-dihydrocoumarin + H2O
-
 Acinetobacter calcoaceticus F46 3-(2-hydroxyphenyl)propionic acid
-
 ?
3.1.1.35 dihydrocoumarin + H2O
-
 Acinetobacter calcoaceticus melilotate
-
 ?
3.1.1.35 dihydrocoumarin + H2O
-
 Acinetobacter calcoaceticus F46 melilotate
-
 ?
3.1.1.35 DL-beta-acetylthioisobutyrate methyl ester + H2O stereospecific hydrolysis Acinetobacter calcoaceticus D-beta-acetylthioisobutyrate + methanol
-
 ?
3.1.1.35 DL-beta-acetylthioisobutyrate methyl ester + H2O stereospecific hydrolysis Acinetobacter calcoaceticus F46 D-beta-acetylthioisobutyrate + methanol
-
 ?
3.1.1.35 peracetic acid peracetic acid is likely a natural substrate in vivo, enzyme may play a role in oxidative stress defense system Acinetobacter calcoaceticus acetic acid + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.35 3,4-dihydrocoumarin hydrolase
-
 Acinetobacter calcoaceticus
3.1.1.35 DCH
-
 Acinetobacter calcoaceticus