EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.3 | expression in Aspergillus oryzae | Thermothielavioides terrestris |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.3 | 0.33 | - |
maltotriose | 20°C, pH 4.0 | Thermothielavioides terrestris |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 62000 | - |
x * 62000, deduced from nucleotide sequence | Thermothielavioides terrestris |
3.2.1.3 | 75000 | - |
x * 75000, SDS-PAGE | Thermothielavioides terrestris |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.3 | maltodextrin + H2O | Thermothielavioides terrestris | - |
beta-D-glucose + ? | - |
? | |
3.2.1.3 | maltose + H2O | Thermothielavioides terrestris | - |
beta-D-glucose + D-glucose | - |
? | |
3.2.1.3 | maltotriose + 2 H2O | Thermothielavioides terrestris | - |
3 beta-D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.3 | Thermothielavioides terrestris | - |
soil-borne thermophilic fungus, synonym, allescheria terrestris | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.3 | glycoprotein | five potential sites for N-glycosylation | Thermothielavioides terrestris |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.3 | recombinant tGA | Thermothielavioides terrestris |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 2.8 | - |
with maltose | Thermothielavioides terrestris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.3 | maltodextrin + H2O | - |
Thermothielavioides terrestris | beta-D-glucose + ? | - |
? | |
3.2.1.3 | maltose + H2O | - |
Thermothielavioides terrestris | beta-D-glucose + D-glucose | - |
? | |
3.2.1.3 | maltotriose + 2 H2O | - |
Thermothielavioides terrestris | 3 beta-D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.3 | ? | x * 75000, SDS-PAGE | Thermothielavioides terrestris |
3.2.1.3 | ? | x * 62000, deduced from nucleotide sequence | Thermothielavioides terrestris |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.3 | tGA | - |
Thermothielavioides terrestris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 60 | - |
with maltotriose | Thermothielavioides terrestris |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 60 | - |
no loss of activity after 30 min | Thermothielavioides terrestris |
3.2.1.3 | 70 | - |
30-40% loss of activity after 10 min, approx. 90% loss of activity after 40 min, complete loss of activity after 60 min | Thermothielavioides terrestris |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.3 | 91.7 | - |
maltotriose | 20°C, pH 4.0 | Thermothielavioides terrestris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 4 | - |
with maltotriose | Thermothielavioides terrestris |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 3 | 8 | approx. 70% of maximal activity at pH 4.0, approx. 5% of maximal activity at pH 8.0 | Thermothielavioides terrestris |