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Literature summary extracted from

  • Gangar, A.; Karande, A.A.; Rajasekharan, R.
    Purification and characterization of acyl-acyl carrier protein synthetase from oleaginous yeast and its role in triacylglycerol biosynthesis (2001), Biochem. J., 360, 471-479.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.20 0.023
-
 linoleic acid pH 8.0, 30°C Rhodotorula glutinis
6.2.1.20 0.025
-
 oleic acid pH 8.0, 30°C Rhodotorula glutinis
6.2.1.20 0.03
-
 stearic acid pH 8.0, 30°C Rhodotorula glutinis
6.2.1.20 0.043
-
 palmitic acid pH 8.0, 30°C Rhodotorula glutinis
6.2.1.20 2.5
-
 ATP pH 8.0, 30°C Rhodotorula glutinis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.20 cytosol
-
 Rhodotorula glutinis 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.20 35000
-
 SDS-PAGE and native PAGE Rhodotorula glutinis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.20 ATP + an acid + [acyl-carrier protein] Rhodotorula glutinis synthesis of activated acyl-carrier protein that is essential as a carrier of acyl-intermediates and donor for activated acyl groups for the synthesis of lipids and lipid derivatives AMP + diphosphate + acyl-[acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.20 Rhodotorula glutinis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.20
-
 Rhodotorula glutinis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.20 ATP + an acid + [acyl-carrier protein]
-
 Rhodotorula glutinis AMP + diphosphate + acyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + an acid + [acyl-carrier protein] synthesis of activated acyl-carrier protein that is essential as a carrier of acyl-intermediates and donor for activated acyl groups for the synthesis of lipids and lipid derivatives Rhodotorula glutinis AMP + diphosphate + acyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + linoleic acid + [acyl-carrier protein] most effective subtrate Rhodotorula glutinis AMP + diphosphate + linoleoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + oleic acid + [acyl-carrier protein]
-
 Rhodotorula glutinis AMP + diphosphate + oleoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + palmitic acid + [acyl-carrier protein]
-
 Rhodotorula glutinis AMP + diphosphate + palmitoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + stearic acid + [acyl-carrier protein]
-
 Rhodotorula glutinis AMP + diphosphate + stearoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 additional information no activity with GTP, CTP or UTP Rhodotorula glutinis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.20 monomer 1 * 35000, molecular weight of native protein and subunits by native and SDS-PAGE Rhodotorula glutinis