Any feedback?
Literature summary extracted from
- Cloning of the xylitol dehydrogenase gene from Gluconobacter oxydans and improved production of xylitol from D-arabitol (2003), Biosci. Biotechnol. Biochem., 67, 584-591.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.1.1.9 | biotechnology | strain overexpressing enzyme has improved xylitol productivity, production of up to 57g/l xylitol from 225 g/l D-arabitol, via D-xylulose | Gluconobacter oxydans |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.9 | - |
Gluconobacter oxydans |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.1.1.9 | additional information | strain overexpressing enzyme has improved xylitol productivity, production of up to 57g/l xylitol from 225 g/l D-arabitol, via D-xylulose | Gluconobacter oxydans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.9 | CaCl2 | 1 mM, 70% inhibition | Gluconobacter oxydans |  |
| 1.1.1.9 | additional information | not inhibitory: MgCl2, ZnCl2, CoCl2, CuCl2 at 1 mM, EDTA at 10 mM | Gluconobacter oxydans | |
| 1.1.1.9 | NiCl2 | 1 mM, complete inhibition | Gluconobacter oxydans | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.9 | cytoplasm | - |
Gluconobacter oxydans | 5737 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.9 | 27000 | - |
x * 27800, deduced from genen sequence, x * 27000, SDS-PAGE | Gluconobacter oxydans |
| 1.1.1.9 | 27800 | - |
x * 27800, deduced from genen sequence, x * 27000, SDS-PAGE | Gluconobacter oxydans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.9 | Gluconobacter oxydans | - |
strain ATCC 621 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.9 | - |
Gluconobacter oxydans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.9 | 113 | - |
substrate D-xylitol, pH 10, 30°C | Gluconobacter oxydans |
| 1.1.1.9 | 220 | - |
substrate D-sorbitol, pH 10, 30°C | Gluconobacter oxydans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.9 | D-sorbitol + NAD+ | - |
Gluconobacter oxydans | D-fructose + NADH + H+ | - |
r | |
| 1.1.1.9 | D-xylitol + NAD+ | - |
Gluconobacter oxydans | D-xylulose + NADH + H+ | - |
r | |
| 1.1.1.9 | D-xylulose + NADH + H+ | - |
Gluconobacter oxydans | D-xylitol + NAD+ | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.9 | ? | x * 27800, deduced from genen sequence, x * 27000, SDS-PAGE | Gluconobacter oxydans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.9 | 5 | - |
reduction of D-xylulose | Gluconobacter oxydans |
| 1.1.1.9 | 11 | - |
oxidation of xylitol | Gluconobacter oxydans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.9 | NAD+ | - |
Gluconobacter oxydans | |
| 1.1.1.9 | NADH | - |
Gluconobacter oxydans | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
