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Literature summary extracted from
- Specificity of human alcohol dehydrogenase 1C*2 (gamma2gamma2) for steroids and simulation of the uncompetitive inhibition of ethanol metabolism (2003), Chem. Biol. Interact., 143-144, 183-193.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.1 | expression of ADH1C*2 in Escherichia coli | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.1 | 3-butylthiolan 1-oxide | dead-end inhibitor to the enzyme-cofactor complex, inhibition of oxidation reaction | Homo sapiens |  |
| 1.1.1.1 | 4-androsten-3,17-dione | competitive against substrate cyclohexanone | Homo sapiens | |
| 1.1.1.1 | 5alpha-androstan-17beta-ol-3-one | i.e. 5alpha-dihydrotestosterone, allosteric, competitive against substrate cyclohexanone, noncompetitive against NAD+ nd ethanol | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.1 | additional information | - |
additional information | steady-state kinetics | Homo sapiens | |
| 1.1.1.1 | 0.003 | - |
5beta-androstan-3beta-ol-17-one | pH 7.3, 37°C, recombinant enzyme | Equus caballus | |
| 1.1.1.1 | 0.0036 | - |
5alpha-androstan-17beta-ol-3-one | pH 7.3, 37°C, ADH1C*2 (gamma2gamma2) | Homo sapiens | |
| 1.1.1.1 | 0.0036 | - |
5beta-pregnan-3,20-dione | pH 7.3, 37°C, ADH1C*2 (gamma2gamma2) | Homo sapiens | |
| 1.1.1.1 | 0.008 | - |
5beta-cholanic acid-3-one | pH 7.3, 37°C, recombinant enzyme | Equus caballus | |
| 1.1.1.1 | 0.011 | - |
5beta-androstan-17beta-ol-3-one | pH 7.3, 37°C, recombinant enzyme | Equus caballus | |
| 1.1.1.1 | 0.027 | - |
5beta-Pregnan-3beta-ol-20-one | pH 7.3, 37°C, ADH1C*2 (gamma2gamma2) | Homo sapiens | |
| 1.1.1.1 | 0.046 | - |
5beta-androstan-17beta-ol-3-one | pH 7.3, 37°C, ADH1C*2 (gamma2gamma2) | Homo sapiens | |
| 1.1.1.1 | 0.058 | - |
5beta-androstan-3beta-ol-17-one | pH 7.3, 37°C, ADH1C*2 (gamma2gamma2) | Homo sapiens | |
| 1.1.1.1 | 0.25 | - |
5beta-cholanic acid-3-one | pH 7.3, 37°C, ADH1C*2 (gamma2gamma2) | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.1 | ethanol + NAD+ | Homo sapiens | - |
acetaldehyde + NADH | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.1 | Equus caballus | - |
- |
- |
| 1.1.1.1 | Homo sapiens | - |
isozyme 1C*2 (gamma2gamma2) | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.1 | liver | - |
Equus caballus | - |
| 1.1.1.1 | liver | isozyme ADH1C*2 | Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.1 | 5alpha-androstan-17beta-ol-3-one + NADH + H+ | - |
Homo sapiens | 3beta,17beta-dihydroxy-5alpha-androstan + NAD+ | - |
? | |
| 1.1.1.1 | 5alpha-pregnan-3beta-ol-20-one + NAD+ | low activity | Homo sapiens | 5alpha-pregnan-3,20-dione + NADH | - |
? | |
| 1.1.1.1 | 5beta-androstan-17beta-ol-3-one + NAD+ | - |
Equus caballus | 5beta-androstan-3,17-dione + NADH | - |
? | |
| 1.1.1.1 | 5beta-androstan-17beta-ol-3-one + NAD+ | low activity | Homo sapiens | 5beta-androstan-3,17-dione + NADH | - |
? | |
| 1.1.1.1 | 5beta-androstan-3beta-ol-17-one + NAD+ | - |
Homo sapiens | 5beta-androstan-3,17-dione + NADH | - |
? | |
| 1.1.1.1 | 5beta-androstan-3beta-ol-17-one + NAD+ | - |
Equus caballus | 5beta-androstan-3,17-dione + NADH | - |
? | |
| 1.1.1.1 | 5beta-cholanic acid-3-one + NADH | low activity | Homo sapiens | 5beta-cholanic acid-3-ol + NAD+ | - |
? | |
| 1.1.1.1 | 5beta-cholanic acid-3-one + NADH + H+ | - |
Equus caballus | 5beta-cholanic acid-3-ol + NAD+ | - |
? | |
| 1.1.1.1 | 5beta-pregnan-3,20-dione + NADH | - |
Homo sapiens | ? | - |
? | |
| 1.1.1.1 | 5beta-pregnan-3beta-ol-20-one + NAD+ | - |
Homo sapiens | 5beta-pregnan-3,20-dione + NADH | - |
? | |
| 1.1.1.1 | cyclohexanone + NADH + H+ | - |
Homo sapiens | cyclohexanol + NAD+ | - |
? | |
| 1.1.1.1 | ethanol + NAD+ | - |
Homo sapiens | acetaldehyde + NADH | - |
? | |
| 1.1.1.1 | ethanol + NAD+ | - |
Equus caballus | acetaldehyde + NADH | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.1 | ADH1C*1 | - |
Homo sapiens |
| 1.1.1.1 | ADH1C*2 | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.1 | 30 | 37 | assay at | Homo sapiens |
| 1.1.1.1 | 30 | 37 | assay at | Equus caballus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.1 | 7.3 | - |
assay at | Homo sapiens |
| 1.1.1.1 | 7.3 | - |
assay at | Equus caballus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.1 | NAD+ | - |
Homo sapiens | |
| 1.1.1.1 | NAD+ | - |
Equus caballus | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.1 | additional information | - |
additional information | inhibition kinetics | Homo sapiens | |
| 1.1.1.1 | 0.0047 | - |
5alpha-androstan-17beta-ol-3-one | pH 7.3, 37°C, versus cyclohexanone | Homo sapiens | |
| 1.1.1.1 | 0.0047 | - |
4-androsten-3,17-dione | pH 7.3, 37°C, versus cyclohexanone | Homo sapiens | |
| 1.1.1.1 | 0.014 | - |
5alpha-androstan-17beta-ol-3-one | pH 7.3, 37°C, versus ethanol | Homo sapiens | |
| 1.1.1.1 | 0.028 | - |
5alpha-androstan-17beta-ol-3-one | pH 7.3, 37°C, versus NAD+ | Homo sapiens | |
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