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Literature summary extracted from

  • Heiden, S.; Hedderich, R.; Setzke, E.; Thauer, R.K.
    Purification of a cytochrome b containing H2:heterodisulfide oxidoreductase complex from membranes of Methanosarcina barkeri (1993), Eur. J. Biochem., 213, 529-535.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.98.1 membrane bound to Methanosarcina barkeri 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.98.1 Iron the H2:heterodisulfide oxidoreductase multienzyme complex contains 70-80 nmol non-heme iron and acid labile sulfur per mg of protein Methanosarcina barkeri
1.8.98.1 Ni the H2:heterodisulfide oxidoreductase multienzyme complex contains 0.6 nmol Ni per mg of protein Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.98.1 additional information
-
 MW of H2:heterodisulfide oxidoreductase multienzyme complex is 800000-1300000, gel filtration Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 Methanosarcina barkeri energy-conserving step in methanohenic archaea coenzyme B + coenzyme M
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.98.1 Methanosarcina barkeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.98.1
-
 Methanosarcina barkeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.98.1 6
-
 reduction of the heterodisulfide with H2 Methanosarcina barkeri
1.8.98.1 24
-
 reduction of the heterodisulfide with benzylviologen Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2
-
 Methanosarcina barkeri coenzyme B + coenzyme M
-
 ?
1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 energy-conserving step in methanohenic archaea Methanosarcina barkeri coenzyme B + coenzyme M
-
 ?
1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced benzyl viologen
-
 Methanosarcina barkeri coenzyme B + coenzyme M + benzyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.98.1 ? the H2:heterodisulfide oxidoreductase multienzyme complex is composed of nine polypepetides with MW 46000 Da, 39000 Da, 28000 Da, 25000 Da, 23000 Da, 21000 Da, 20000 Da, 16000 Da, 15000 Da, SDS-PAGE Methanosarcina barkeri

Synonyms

EC Number Synonyms Comment Organism
1.8.98.1 H2:heterodisulfide oxidoreductase
-
 Methanosarcina barkeri
1.8.98.1 More the H2:heterodisulfide oxidoreductase multienzyme complex is composed of nine polypeptides with MW 46000 Da, 39000 Da, 28000 Da, 25000 Da, 23000 Da, 21000 Da, 20000 Da, 16000 Da, 15000 Da Methanosarcina barkeri

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.98.1 cytochrome b the H2:heterodisulfide oxidoreductase multienzyme complex contains 3.2 nmol cytochrome b per mg protein Methanosarcina barkeri
1.8.98.1 FAD the H2:heterodisulfide oxidoreductase multienzyme complex contiams 0.6 nmol per mg of protein Methanosarcina barkeri
1.8.98.1 additional information the H2:heterodisukfide oxidoreductase complex does not mediate the reduction of coenzyme F420 with H2 nor the oxidation of reduced coenzyme F420 with the heterodisulfide Methanosarcina barkeri