EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.1.2 | H2 + NAD+ | Frankia sp. R43 | H2 producing activity is not dependent on NAD+ reduction | H+ + NADH | - |
r | |
1.12.1.2 | H2 + oxidized methyl viologen | Frankia sp. R43 | the artificial electron donor is accepted by the cells for H2 production when added to the culture medium | H+ + reduced methyl viologen | - |
r | |
1.12.1.2 | additional information | Frankia sp. R43 | enzyme is the key enzyme in hydrogen metabolism, no activity in strain R43 under aerobic nitrogen-limited conditions | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.1.2 | Frankia sp. R43 | P22317 | hoxF, alpha-subunit; LLR02022, nitrogen-fixing actinomycete, no activity in strain KB5 | - |
1.12.1.2 | Frankia sp. R43 | P22318 | hoxU, gamma-subunit; LLR02022, nitrogen-fixing actinomycete, no activity in strain KB5 | - |
1.12.1.2 | Frankia sp. R43 | P22319 | hoxY, delta-subunit; LLR02022, nitrogen-fixing actinomycete, no activity in strain KB5 | - |
1.12.1.2 | Frankia sp. R43 | P22320 | hoxH, beta-subunit; LLR02022, nitrogen-fixing actinomycete, no activity in strain KB5 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.12.1.2 | hypha | - |
Frankia sp. R43 | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.12.1.2 | additional information | - |
H2-uptake activity at different conditions | Frankia sp. R43 |
1.12.1.2 | 0.0006 | - |
cell culture in nitrogen-enriched medium, anaerobic conditions, H2 production using reduced methyl viologen as electron donor | Frankia sp. R43 |
1.12.1.2 | 0.0018 | - |
cell culture in nitrogen-limited medium, anaerobic conditions, H2 production using reduced methyl viologen as electron donor | Frankia sp. R43 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.1.2 | H2 + NAD+ | - |
Frankia sp. R43 | H+ + NADH | - |
r | |
1.12.1.2 | H2 + NAD+ | H2 producing activity is not dependent on NAD+ reduction | Frankia sp. R43 | H+ + NADH | - |
r | |
1.12.1.2 | H2 + oxidized methyl viologen | - |
Frankia sp. R43 | H+ + reduced methyl viologen | - |
r | |
1.12.1.2 | H2 + oxidized methyl viologen | the artificial electron donor is accepted by the cells for H2 production when added to the culture medium | Frankia sp. R43 | H+ + reduced methyl viologen | - |
r | |
1.12.1.2 | additional information | enzyme is the key enzyme in hydrogen metabolism, no activity in strain R43 under aerobic nitrogen-limited conditions | Frankia sp. R43 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.12.1.2 | hydrogen-evolving enzyme | - |
Frankia sp. R43 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.1.2 | 27 | - |
assay at, free living cell culture in vivo | Frankia sp. R43 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.12.1.2 | 7 | - |
assay at, free living cell culture in vivo | Frankia sp. R43 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.12.1.2 | additional information | methyl viologen can replace NAD+/NADH | Frankia sp. R43 | |
1.12.1.2 | NAD+ | - |
Frankia sp. R43 | |
1.12.1.2 | NADH | - |
Frankia sp. R43 |