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Literature summary extracted from

  • de Barros, J.F., Jr.; Sales Alviano, D.; da Silva, M.H.; Dutra Wigg, M.; Sales Alviano, C.; Schauer, R.; dos Santos Silva Couceiro, J.N.
    Characterization of sialidase from an influenza A (H3N2) virus strain: kinetic parameters and substrate specificity (2003), Intervirology, 46, 199-206.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.18 0.16
-
 2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid pH 5.2 influenza A virus
3.2.1.18 0.18
-
 alpha-(2-3)-sialyllactose pH 5.2 influenza A virus
3.2.1.18 0.52
-
 alpha-(2-6)-sialyllactose pH 5.2 influenza A virus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.18 Ca2+ activates influenza A virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.18 200000 240000
-
 influenza A virus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.18 bovine brain gangliosides + H2O influenza A virus
-
 sialic acid + ?
-
 ?
3.2.1.18 bovine submaxillary gland mucin + H2O influenza A virus
-
 sialic acid + ?
-
 ?
3.2.1.18 colominic acid + H2O influenza A virus
-
 sialic acid + ?
-
 ?
3.2.1.18 fetuin + H2O influenza A virus
-
 sialic acid + ?
-
 ?
3.2.1.18 orosomucoid + H2O influenza A virus
-
 sialic acid + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.18 influenza A virus
-
 H3N2, strain X-31, reassorting of influenza A/Victoria/3/75 and influenza A/PR/8/34, from inoculated chicken egg allantoic fluid
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.18 from inoculated chicken egg allantoic fluid, by ultracentrifugation, gel filtration and proteolytic treatment with pronase, soluble 'head' part of the enzyme complex influenza A virus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.18 0.15
-
 purified enzyme, substrate alpha-(2-6)-sialyllactose influenza A virus
3.2.1.18 2.13
-
 purified enzyme, substrate alpha-(2-3)-sialyllactose influenza A virus
3.2.1.18 11.58
-
 purified enzyme, substrate 2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid influenza A virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.18 2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
-
 influenza A virus 4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
 ?
3.2.1.18 alpha(2-3)-sialyllactose + H2O preference for alpha(2-3)-linkages influenza A virus sialic acid + lactose
-
 ?
3.2.1.18 alpha(2-6)-sialyllactose + H2O
-
 influenza A virus sialic acid + lactose
-
 ?
3.2.1.18 bovine brain gangliosides + H2O
-
 influenza A virus sialic acid + ?
-
 ?
3.2.1.18 bovine submaxillary gland mucin + H2O
-
 influenza A virus sialic acid + ?
-
 ?
3.2.1.18 Candida albicans cells + H2O
-
 influenza A virus desialylated Candida albicans cells + sialic acid
-
 ?
3.2.1.18 colominic acid + H2O
-
 influenza A virus sialic acid + ?
-
 ?
3.2.1.18 fetuin + H2O
-
 influenza A virus sialic acid + ?
-
 ?
3.2.1.18 additional information enzyme agglutinates erythrocytes from human blood influenza A virus ?
-
 ?
3.2.1.18 orosomucoid + H2O
-
 influenza A virus sialic acid + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.18 More enzyme possesses 2 distinct regions, a globular one, exposed to the exterior, and a stem portion, part of which is inserted into the virion envelope influenza A virus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.18 NA
-
 influenza A virus
3.2.1.18 neuraminidase
-
 influenza A virus
3.2.1.18 sialidase
-
 influenza A virus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.18 37
-
 assay at influenza A virus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.18 56
-
 inactivation influenza A virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.18 4.4
-
 with substrate alpha-(2-6)-sialyllactose influenza A virus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.18 4 6.8
-
 influenza A virus