EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.10 | construction of a cDNA library, DNA sequence determination and analysis, subcloning in Escherichia coli DH5alpha, overexpression as His-tagged enzyme in Saccharomyces cerevisiae strain CEN.PK2 | Ambrosiozyma monospora |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.10 | 2.7 | - |
D-arabinitol | pH 9.0, 30°C, purified recombinant enzyme | Ambrosiozyma monospora | |
1.1.1.10 | 4.7 | - |
D-ribulose | pH 7.0, 30°C, purified recombinant enzyme | Ambrosiozyma monospora | |
1.1.1.10 | 7.2 | - |
L-xylitol | pH 9.0, 30°C, purified recombinant enzyme | Ambrosiozyma monospora | |
1.1.1.10 | 9.6 | - |
L-xylulose | pH 7.0, 30°C, purified recombinant enzyme | Ambrosiozyma monospora |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.10 | Mg2+ | - |
Ambrosiozyma monospora |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.10 | L-xylulose + NADH | Ambrosiozyma monospora | part of the L-arabinose catabolism | L-xylitol + NAD+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.10 | Ambrosiozyma monospora | Q70FD1 | fragment; yeast, strain NRRL Y-1484 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.10 | recombinant His-tagged enzyme from Saccharomyces cerevisiae by nickel affinity chromatography | Ambrosiozyma monospora |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.10 | D-ribulose + NADH | - |
Ambrosiozyma monospora | D-arabinitol + NAD+ | - |
r | |
1.1.1.10 | D-xylulose + NADH + H+ | about 100fold lower activity compared to L-xylulose | Ambrosiozyma monospora | D-arabinitol + NAD+ | - |
r | |
1.1.1.10 | L-xylulose + NADH | - |
Ambrosiozyma monospora | L-xylitol + NAD+ | - |
r | |
1.1.1.10 | L-xylulose + NADH | part of the L-arabinose catabolism | Ambrosiozyma monospora | L-xylitol + NAD+ | - |
r | |
1.1.1.10 | additional information | NADH-linked enzyme form, no activity with NADPH and NADP+, no activity with L-arabinitol, adonitol or ribitol, dulcitol or galactitol, D-mannitol, and D-sorbitol, no activity with D-sorbose, L-sorbose, D-psicose, and D-fructose | Ambrosiozyma monospora | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.10 | 30 | - |
assay at | Ambrosiozyma monospora |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.10 | 7 | - |
forward, reduction reaction | Ambrosiozyma monospora |
1.1.1.10 | 9 | - |
reverse, oxidation reaction | Ambrosiozyma monospora |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.10 | NAD+ | specific for, no activity with NADP+ | Ambrosiozyma monospora | |
1.1.1.10 | NADH | specific for no activity with NADPH | Ambrosiozyma monospora |