EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.12.1.2 | FMN | activates | Caldanaerobacter subterraneus subsp. tengcongensis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.12.1.2 | 0.017 | - |
NADH | 70°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
1.12.1.2 | 0.09 | - |
NAD+ | 70°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
1.12.7.2 | 0.003 | - |
reduced ferredoxin | 70°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
1.12.7.2 | 0.015 | - |
oxidized ferredoxin | 70°C | Caldanaerobacter subterraneus subsp. tengcongensis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.12.1.2 | soluble | - |
Caldanaerobacter subterraneus subsp. tengcongensis | - |
- |
1.12.7.2 | membrane | membrane bound | Caldanaerobacter subterraneus subsp. tengcongensis | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.12.1.2 | Iron | enzyme contains multiple iron-sulfur clusters, [4Fe-4S] or [2Fe-2S] | Caldanaerobacter subterraneus subsp. tengcongensis | |
1.12.1.2 | Ni2+ | below 0.06 mol Ni2+ per mol of enzyme | Caldanaerobacter subterraneus subsp. tengcongensis | |
1.12.7.2 | Fe | NiFe hydrogenase | Caldanaerobacter subterraneus subsp. tengcongensis | |
1.12.7.2 | Ni | NiFe hydrogenase, contains 0.9 mol Ni per mol enzyme | Caldanaerobacter subterraneus subsp. tengcongensis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.12.1.2 | 14000 | - |
1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.1.2 | 20000 | - |
1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.1.2 | 64000 | - |
1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.1.2 | 65000 | - |
1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.1.2 | 162500 | - |
amino acid sequence calculation | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.7.2 | 14700 | - |
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.7.2 | 17000 | - |
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.7.2 | 32000 | - |
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.7.2 | 40000 | - |
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.7.2 | 69000 | - |
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.1.2 | H2 + NAD+ | Caldanaerobacter subterraneus subsp. tengcongensis | - |
H+ + NADH | - |
r | |
1.12.1.2 | additional information | Caldanaerobacter subterraneus subsp. tengcongensis | at high H2 partial pressure enzyme activity is reduced and fermentation is partially shifted to ethanol production | ? | - |
? | |
1.12.7.2 | H+ + reduced ferredoxin | Caldanaerobacter subterraneus subsp. tengcongensis | - |
H2 + oxidized ferredoxin | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.1.2 | Caldanaerobacter subterraneus subsp. tengcongensis | - |
- |
- |
1.12.7.2 | Caldanaerobacter subterraneus subsp. tengcongensis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.12.1.2 | 20fold by several chromatographic steps | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.7.2 | Q-sepharose HiLoad, hydroxyapatite, Superdex 200 | Caldanaerobacter subterraneus subsp. tengcongensis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.12.1.2 | additional information | activity is high in cells grown at low H2 partial pressure | Caldanaerobacter subterraneus subsp. tengcongensis | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.12.1.2 | 0.6 | - |
H2-evolution in vivo, fermenter-grown cells | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.1.2 | 5 | - |
purified enzyme, H2 consumption | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.1.2 | 10 | - |
purified enzyme, H2 production | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.1.2 | 1300 | - |
purified enzyme, with substrates oxidized benzyl viologen and NADH | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.1.2 | 1700 | - |
purified enzyme, both reaction directions with substrates oxidized/reduced methyl viologen and H2/H+ | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.7.2 | 38.5 | - |
reduction of metronidiazole | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.7.2 | 391 | - |
reduction of methyl viologen | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.7.2 | 1260 | - |
oxidation of methyl viologen | Caldanaerobacter subterraneus subsp. tengcongensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.1.2 | H2 + NAD+ | - |
Caldanaerobacter subterraneus subsp. tengcongensis | H+ + NADH | - |
r | |
1.12.1.2 | H2 + NAD+ | electron transfer between the catalytic site for NADH-oxidation and the hydrogenase catalytic site is rate-limiting | Caldanaerobacter subterraneus subsp. tengcongensis | H+ + NADH | - |
r | |
1.12.1.2 | H2 + oxidized methyl viologen | - |
Caldanaerobacter subterraneus subsp. tengcongensis | H+ + reduced methyl viologen | - |
r | |
1.12.1.2 | additional information | at high H2 partial pressure enzyme activity is reduced and fermentation is partially shifted to ethanol production | Caldanaerobacter subterraneus subsp. tengcongensis | ? | - |
? | |
1.12.1.2 | additional information | enzyme autoxidation | Caldanaerobacter subterraneus subsp. tengcongensis | ? | - |
? | |
1.12.1.2 | NADH + oxidized benzyl viologen | - |
Caldanaerobacter subterraneus subsp. tengcongensis | NAD+ + reduced benzyl viologen | - |
r | |
1.12.7.2 | H+ + reduced ferredoxin | - |
Caldanaerobacter subterraneus subsp. tengcongensis | H2 + oxidized ferredoxin | - |
r | |
1.12.7.2 | H+ + reduced methyl viologen | - |
Caldanaerobacter subterraneus subsp. tengcongensis | H2 + oxidized methyl viologen | - |
r | |
1.12.7.2 | H2 + ferredoxin + oxidized metronidazole | - |
Caldanaerobacter subterraneus subsp. tengcongensis | H+ + ferredoxin + reduced metronidazole | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.12.1.2 | tetramer | 1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.7.2 | ? | x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.12.1.2 | NADH-dependent Fe-only hydrogenase | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
1.12.7.2 | Ech hydrogenase | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.1.2 | 70 | - |
assay at | Caldanaerobacter subterraneus subsp. tengcongensis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.12.1.2 | FMN | required, enzyme contains 1 mol FMN per mol of enzyme | Caldanaerobacter subterraneus subsp. tengcongensis | |
1.12.1.2 | additional information | methyl viologen or benzyl viologen can replace NAD+/NADH | Caldanaerobacter subterraneus subsp. tengcongensis | |
1.12.1.2 | additional information | no activity with NADPH/NADP+ | Caldanaerobacter subterraneus subsp. tengcongensis | |
1.12.1.2 | NAD+ | highly specific for | Caldanaerobacter subterraneus subsp. tengcongensis | |
1.12.1.2 | NADH | highly specific for | Caldanaerobacter subterraneus subsp. tengcongensis |