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Literature summary extracted from

  • Wang, S.H.; Kuo, S.C.; Chen, S.C.
    High-performance liquid chromatography determination of methionine adenosyltransferase activity using catechol-O-methyltransferase-coupled fluorometric detection (2003), Anal. Biochem., 319, 13-20.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.6 analysis high-performance liquid chromatography determination of methionine adenosyltransferase activity using catechol-O-methyltransferase-coupled fluorometric detection, a nonradioactive, sensitive, rapid, and specific method for the determination of methionine adenosyltransferase activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.6 0.0061
-
 S-adenosyl-L-methionine
-
 Homo sapiens
2.5.1.6 additional information
-
 additional information high performance liquid chromatography assay method using catechol-O-methyltransferase-coupled fluorometric detection, negative cooperativity of enzyme with Hill coefficinet of 0.5 Homo sapiens
2.5.1.6 0.0061
-
 L-methionine pH 7.0, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.6 Homo sapiens
-
-
-
2.5.1.6 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.6 HL-60 cell
-
 Homo sapiens
-
2.5.1.6 HL-60 cell leukemia promyelotic cells Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.6 216
-
 pH 7.0, 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.6 S-adenosyl-L-methionine + esculetin
-
 Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.6 S-adenosyl-L-methionine + scopoletin
-
 Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
2.5.1.6 ATP + L-methionine + H2O
-
 Homo sapiens phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?