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Literature summary extracted from

  • Naranjo, L.; Martin de Valmaseda, E.; Casqueiro, J.; Ullan, R.V.; Lamas-Maceiras, M.; Banuelos, O.; Martin, J.F.
    Inactivation of the lys7 gene, encoding saccharopine reductase in Penicillium chrysogenum, leads to accumulation of the secondary metabolite precursors piperideine-6-carboxylic acid and pipecolic acid from alpha-aminoadipic acid (2004), Appl. Environ. Microbiol., 70, 1031-1039.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.10 gene lys, subcloning in Escherichia coli Penicillium chrysogenum

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.10 additional information construction of an enzyme-deficient mutant strain: disruption and inactivation of gene lys7 by double-recombination method leads to lysine auxotrophy and accumulation of piperideine-6-carboxylic acid, and with L-lysine as sole N-source and supplementation of DL-alpha-aminoadipic acid, also of pipecolic acid, transformation of the mutant strain with lys7 can restore enzyme activity Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.10 L-glutamate + 2-aminoadipate 6-semialdehyde + NADPH + H+ Penicillium chrysogenum piperidine-6-carboxylic acid and alpha-aminoadipate are precursors for synthesis of alpha-aminoadipate 6-semialdehyde via 3 different pathways, penultimate step in L-lysine biosynthesis saccharopine + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.10 Penicillium chrysogenum
-
 gene lys7, strain Wis 54-1255
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.10 22
-
 wild-type strain Wis 54-1255, cell extract Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.10 L-glutamate + 2-aminoadipate 6-semialdehyde + NADPH + H+ piperidine-6-carboxylic acid and alpha-aminoadipate are precursors for synthesis of alpha-aminoadipate 6-semialdehyde via 3 different pathways, penultimate step in L-lysine biosynthesis Penicillium chrysogenum saccharopine + NADP+ + H2O
-
 ?
1.5.1.10 saccharopine + NADP+ + H2O reverse reaction direction used for activity assay, end product is piperidine-6-carboxylic acid in assays using cell extract Penicillium chrysogenum L-glutamate + 2-aminoadipate 6-semialdehyde + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.5.1.10 saccharopine reductase
-
 Penicillium chrysogenum
1.5.1.10 SR1
-
 Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.10 30
-
 assay at Penicillium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.10 9
-
 assay at Penicillium chrysogenum

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.10 NADP+
-
 Penicillium chrysogenum
1.5.1.10 NADPH
-
 Penicillium chrysogenum