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Literature summary extracted from

  • Okuta, A.; Ohnishi, K.; Yagame, S.; Harayama, S.
    Intersubunit interaction and catalytic activity of catechol 2,3-dioxygenases (2003), Biochem. J., 371, 557-564.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 expressed in Escherichia coli Pseudomonas sp.
1.13.11.2 expressed in Escherichia coli Sphingomonas sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.2 additional information construction of different hybrid enzymes containing different parts of type I.2.A and I.2.B enzyme, only one of the constructed enzymes is found to be active Pseudomonas sp.
1.13.11.2 additional information construction of different hybrid enzymes containing different parts of type I.2.A and I.2.B enzyme, only one of the constructed enzymes is found to be active Sphingomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.0029
-
 catechol native enzyme, pH 7.5, 25°C Pseudomonas sp.
1.13.11.2 0.0038
-
 catechol native enzyme, pH 7.5, 25°C Sphingomonas sp.
1.13.11.2 0.02
-
 catechol hybrid enzyme, pH 7.5, 25°C Pseudomonas sp.
1.13.11.2 0.02
-
 catechol hybrid enzyme, pH 7.5, 25°C Sphingomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.2 Iron bound to protein Pseudomonas sp.
1.13.11.2 Iron bound to protein Sphingomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.2 37000
-
 4 * 37000, SDS-PAGE Pseudomonas sp.
1.13.11.2 37000
-
 4 * 37000, SDS-PAGE Sphingomonas sp.
1.13.11.2 150000
-
 gel filtration Pseudomonas sp.
1.13.11.2 150000
-
 gel filtration Sphingomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.2 catechol + O2 Pseudomonas sp. key enzyme for the degradation of aromatic compounds 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 Sphingomonas sp. key enzyme for the degradation of aromatic compounds 2-hydroxymuconate semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Pseudomonas sp.
-
 enzyme subgroup I.2.A, hybrid enzymes with type I.2.B enzyme from Sphingomonas sp.
-
1.13.11.2 Sphingomonas sp.
-
 enzyme subgroup I.2.B, hybrid enzymes with type I.2.A enzyme from Pseudomonas sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 catechol + O2
-
 Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Sphingomonas sp. 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 key enzyme for the degradation of aromatic compounds Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 key enzyme for the degradation of aromatic compounds Sphingomonas sp. 2-hydroxymuconate semialdehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.2 tetramer 4 * 37000, SDS-PAGE Pseudomonas sp.
1.13.11.2 tetramer 4 * 37000, SDS-PAGE Sphingomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.2 148
-
 catechol hybrid enzyme, pH 7.5, 25°C Pseudomonas sp.
1.13.11.2 148
-
 catechol hybrid enzyme, pH 7.5, 25°C Sphingomonas sp.
1.13.11.2 238
-
 catechol native enzyme, pH 7.5, 25°C Sphingomonas sp.
1.13.11.2 493
-
 catechol native enzyme, pH 7.5, 25°C Pseudomonas sp.