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Literature summary extracted from

  • Masullo, M.; Cantiello, P.; de Paola, B.; Catanzano, F.; Arcari, P.; Bocchini, V.
    G13A substitution affects the biochemical and physical properties of the elongation factor 1 alpha. A reduced intrinsic GTPase activity is partially restored by kirromycin (2002), Biochemistry, 41, 628-633.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.5.3 kirromycin enhances activity of mutant enzyme G13A (maximal stimulation at 0.04 mM), does not stimulate intrinsic GTPase of SsEF-1alpha triggered by 3.6 M NaCl Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
3.6.5.3 G13A compared to wild-type enzyme the mutant shows a reduced rate of Phe polymerization and a reduced intrinsic GTPase activity that is stimulated by high concentrations of NaCl. Mutant enzyme shows an increased affinity for GTP and GDP. The temperature inducing a 50% denaturation of the mutant enzyme is 5°C lower than that of the wild-type enzyme Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.5.3 GDP
-
 Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.5.3 0.0027
-
 GTP 60°C, wild-type enzyme Saccharolobus solfataricus
3.6.5.3 0.0046
-
 GTP 60°C, mutant enzyme G13A Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.3 Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.5.3 GTP + H2O
-
 Saccharolobus solfataricus GDP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.5.3 elongation factor 1alpha
-
 Saccharolobus solfataricus
3.6.5.3 SsEF-1alpha
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.5.3 80
-
 mutant G13A Saccharolobus solfataricus
3.6.5.3 90
-
 wild-type enzyme Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.6.5.3 70 95 70°C: about 60% of maximal activity, 95°C: about 90% of maximal activity, wild-type enzyme Saccharolobus solfataricus
3.6.5.3 70 86 about 60% of maximal activity at 70°C and at 86°C, mutant enzyme G13A Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.5.3 87
-
 half-denaturation of mutant enzyme G13A Saccharolobus solfataricus
3.6.5.3 91
-
 10 min, 50% inactivation of GDP binding ability, mutant enzyme G13A Saccharolobus solfataricus
3.6.5.3 92
-
 half-denaturation of wild-type enzyme Saccharolobus solfataricus
3.6.5.3 94
-
 10 min, 50% inactivation of GDP binding ability, wild-type enzyme Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.5.3 0.00167
-
 GTP 60°C, mutant enzyme G13A Saccharolobus solfataricus
3.6.5.3 0.013
-
 GTP 60°C, wild-type enzyme Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.5.3 0.0008
-
 GDP 60°C, wild-type enzyme Saccharolobus solfataricus
3.6.5.3 0.0042
-
 GDP 60°C, mutant enzyme G13A Saccharolobus solfataricus