EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.1.1.20 | hanging drop method of vapor diffusion, crystal structure of the enzyme complexed with S-adenosyl-L-methionine and acetate (a potent competitive inhibitor of Gly) and the R175K mutated enzyme complexed with S-adenosyl-L-methionine are determined at 2.8 A and 3.0 A resolution, respectively | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.20 | Y194F | the ratio of turnover-number to KM-value for S-adenosyl-L-methionine is 2.4fold lower than the wild-type value, the ratio of turnover-number to KM-value for Gly is 16.4fold lower than the wild-type value | Rattus norvegicus |
2.1.1.20 | Y21F | the ratio of turnover-number to KM-value for S-adenosyl-L-methionine is 5fold lower than the wild-type value, the ratio of turnover-number to KM-value for Gly is 2.4fold lower than the wild-type value | Rattus norvegicus |
2.1.1.20 | Y220F | the ratio of turnover-number to KM-value for S-adenosyl-L-methionine is nearly identical to the wild-type value, the ratio of turnover-number to KM-value for Gly is 179fold lower than the wild-type value | Rattus norvegicus |
2.1.1.20 | Y242F | the ratio of turnover-number to KM-value for S-adenosyl-L-methionine is 1.14fold higher than the wild-type value, the ratio of turnover-number to KM-value for Gly is 2325fold lower than the wild-type value | Rattus norvegicus |
2.1.1.20 | Y33F | the ratio of turnover-number to KM-value for S-adenosyl-L-methionine is nearly identical to the wild-type value, the ratio of turnover-number to KM-value for Gly is 123fold lower than the wild-type value | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.20 | S-adenosyl-L-homocysteine | weak | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.20 | 0.014 | - |
S-adenosyl-L-methionine | pH 7.2, mutant enzyme Y220F | Rattus norvegicus | |
2.1.1.20 | 0.017 | - |
S-adenosyl-L-methionine | pH 7.2, mutant enzyme Y33F | Rattus norvegicus | |
2.1.1.20 | 0.032 | - |
S-adenosyl-L-methionine | pH 7.2, mutant enzyme Y242F | Rattus norvegicus | |
2.1.1.20 | 0.035 | - |
S-adenosyl-L-methionine | pH 7.2, mutant enzyme Y194F | Rattus norvegicus | |
2.1.1.20 | 0.036 | - |
S-adenosyl-L-methionine | pH 7.2, wild-type enzyme | Rattus norvegicus | |
2.1.1.20 | 0.042 | - |
S-adenosyl-L-methionine | pH 7.2, mutant enzyme Y21F | Rattus norvegicus | |
2.1.1.20 | 0.24 | - |
Gly | pH 7.2, mutant enzyme Y21F | Rattus norvegicus | |
2.1.1.20 | 0.43 | - |
Gly | pH 7.2, wild-type enzyme | Rattus norvegicus | |
2.1.1.20 | 0.44 | - |
Gly | pH 7.2, mutant enzyme Y242F | Rattus norvegicus | |
2.1.1.20 | 2.8 | - |
Gly | pH 7.2, mutant enzyme Y194F | Rattus norvegicus | |
2.1.1.20 | 25 | - |
Gly | pH 7.2, mutant enzyme Y33F | Rattus norvegicus | |
2.1.1.20 | 30 | - |
Gly | pH 7.2, mutant enzyme Y220F | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.20 | Rattus norvegicus | P13255 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.20 | S-adenosyl-L-methionine + glycine | mechanism: the bound S-adenosyl-L-methionine is firmly connected to protein and a Gly pocket" is created near the bound S-adenosyl-L-methionine. The second substrate Gly binds to Arg175 and is brought into the Gly pocket. Five hydrogen bonds connect the Gly in the proximity of the bound S-adenosyl-L-methionine and orient the lone pair orbital on the amino nitrogen of Gly towards the donor methyl group of S-adenosyl-L-methionine. Thermal motion of the enzyme leads to a collision of the N and C(E) so that a SN2 methyltransfer reaction occurs | Rattus norvegicus | S-adenosyl-L-homocysteine + sarcosine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.20 | GNMT | - |
Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.20 | 0.105 | - |
S-adenosyl-L-methionine | pH 7.2, mutant enzyme Y21F | Rattus norvegicus | |
2.1.1.20 | 0.105 | - |
Gly | pH 7.2, mutant enzyme Y21F | Rattus norvegicus | |
2.1.1.20 | 0.18 | - |
S-adenosyl-L-methionine | pH 7.2, mutant enzyme Y194F | Rattus norvegicus | |
2.1.1.20 | 0.18 | - |
Gly | pH 7.2, mutant enzyme Y194F | Rattus norvegicus | |
2.1.1.20 | 0.18 | - |
S-adenosyl-L-methionine | pH 7.2, mutant enzyme Y220F | Rattus norvegicus | |
2.1.1.20 | 0.18 | - |
Gly | pH 7.2, mutant enzyme Y220F | Rattus norvegicus | |
2.1.1.20 | 0.21 | - |
S-adenosyl-L-methionine | pH 7.2, mutant enzyme Y33F | Rattus norvegicus | |
2.1.1.20 | 0.21 | - |
Gly | pH 7.2, mutant enzyme Y33F | Rattus norvegicus | |
2.1.1.20 | 0.45 | - |
S-adenosyl-L-methionine | pH 7.2, wild-type enzyme | Rattus norvegicus | |
2.1.1.20 | 0.45 | - |
Gly | pH 7.2, wild-type enzyme | Rattus norvegicus | |
2.1.1.20 | 0.47 | - |
S-adenosyl-L-methionine | pH 7.2, mutant enzyme Y242F | Rattus norvegicus | |
2.1.1.20 | 0.47 | - |
Gly | pH 7.2, mutant enzyme Y242F | Rattus norvegicus |