Literature summary extracted from

  • Krungkrai, S.R.; Aoki, S.; Palacpac, N.M.; Sato, D.; Mitamura, T.; Krungkrai, J.; Horii, T.
    Human malaria parasite orotate phosphoribosyltransferase: functional expression, characterization of kinetic reaction mechanism and inhibition profile (2004), Mol. Biochem. Parasitol., 134, 245-255.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.10
-
Plasmodium falciparum

General Stability

EC Number General Stability Organism
2.4.2.10 recombinant enzyme, stable for at least 3 months at 4°C or -20°C in presence of 1 mM dithiothreitol and 10% glycerol Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.10 5-aminoorotate 50% inhibition at 0.01 mM Plasmodium falciparum
2.4.2.10 5-bromoorotate
-
Plasmodium falciparum
2.4.2.10 5-Fluoroorotate 50% inhibition at 2-10 nM Plasmodium falciparum
2.4.2.10 5-fluorouracil 50% inhibition at 0.005-0.01 mM Plasmodium falciparum
2.4.2.10 5-iodoorotate
-
Plasmodium falciparum
2.4.2.10 5-Methylorotate 50% inhibition at 0.001 mM Plasmodium falciparum
2.4.2.10 5-phospho-alpha-D-ribose 1-diphosphate product inhibition, competitive to diphosphate, competitive to orotidine 5'-phosphate Plasmodium falciparum
2.4.2.10 diphosphate product inhibition, noncompetitive to orotate, competitive to 5-phospho-alpha-D-ribose 1-diphosphate Plasmodium falciparum
2.4.2.10 Orotate product inhibition, noncompetitive to diphosphate, competitive to orotidine 5'-phosphate Plasmodium falciparum
2.4.2.10 orotidine 5'-phosphate product inhibition, competitive to 5-phospho-alpha-D-ribose 1-diphosphate, competitive to orotate Plasmodium falciparum
2.4.2.10 pyrazofurin 50% inhibition at 0.006-0.024 mM Plasmodium falciparum
2.4.2.10 Uracil
-
Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.10 0.0143
-
diphosphate pH 8.0, 37°C Plasmodium falciparum
2.4.2.10 0.025
-
5-Fluoroorotate pH 8.0, 37°C Plasmodium falciparum
2.4.2.10 0.0182
-
Orotate pH 8.0, 37°C Plasmodium falciparum
2.4.2.10 0.0251
-
orotidine 5'-phosphate pH 8.0, 37°C Plasmodium falciparum
2.4.2.10 0.0282
-
5-phospho-alpha-D-ribose 1-diphosphate pH 8.0, 37°C Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.10 67000
-
gel filtration, recombinant enzyme Plasmodium falciparum
2.4.2.10 33000
-
2 * 33400, recombinant enzyme, SDS-PAGE, 2 * 33000, calculated Plasmodium falciparum
2.4.2.10 33400
-
2 * 33400, recombinant enzyme, SDS-PAGE, 2 * 33000, calculated Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.10 Plasmodium falciparum Q8N0R1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.10 recombinant enzyme Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.10 orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
Plasmodium falciparum orotidine 5'-phosphate + diphosphate
-
r
2.4.2.10 5-fluoroorotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
Plasmodium falciparum ?
-
?

Subunits

EC Number Subunits Comment Organism
2.4.2.10 dimer 2 * 33400, recombinant enzyme, SDS-PAGE, 2 * 33000, calculated Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.10 2.6
-
orotidine 5'-phosphate pH 8.0, 37°C Plasmodium falciparum
2.4.2.10 1.3
-
diphosphate pH 8.0, 37°C Plasmodium falciparum
2.4.2.10 3
-
Orotate pH 8.0, 37°C Plasmodium falciparum
2.4.2.10 3.2
-
5-phospho-alpha-D-ribose 1-diphosphate pH 8.0, 37°C Plasmodium falciparum
2.4.2.10 4
-
5-Fluoroorotate pH 8.0, 37°C Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.10 0.0075
-
Orotate pH 8.0, 37°C, cosubstrate orotidine 5'-phosphate Plasmodium falciparum
2.4.2.10 0.125
-
diphosphate pH 8.0, 37°C, cosubstrate 5-phospho-alpha-D-ribose 1-diphosphate Plasmodium falciparum
2.4.2.10 0.0482
-
5-bromoorotate pH 8.0, 37°C Plasmodium falciparum
2.4.2.10 0.0153
-
5-iodoorotate pH 8.0, 37°C Plasmodium falciparum
2.4.2.10 0.0157
-
orotidine 5'-phosphate pH 8.0, 37°C, cosubstrate 5-phospho-alpha-D-ribose 1-diphosphate Plasmodium falciparum
2.4.2.10 0.0213
-
Orotate pH 8.0, 37°C, cosubstrate diphosphate Plasmodium falciparum
2.4.2.10 0.0423
-
5-phospho-alpha-D-ribose 1-diphosphate pH 8.0, 37°C, cosubstrate diphosphate Plasmodium falciparum
2.4.2.10 0.0241
-
orotidine 5'-phosphate pH 8.0, 37°C, cosubstrate orotate Plasmodium falciparum
2.4.2.10 0.0797
-
diphosphate pH 8.0, 37°C, cosubstrate orotate Plasmodium falciparum
2.4.2.10 0.1501
-
5-phospho-alpha-D-ribose 1-diphosphate pH 8.0, 37°C, cosubstrate orotidine 5'-phosphate Plasmodium falciparum
2.4.2.10 0.0954
-
5-Fluoroorotate pH 8.0, 37°C Plasmodium falciparum
2.4.2.10 0.0214
-
5-Methylorotate pH 8.0, 37°C Plasmodium falciparum
2.4.2.10 0.1212
-
Uracil pH 8.0, 37°C Plasmodium falciparum
2.4.2.10 0.0561
-
5-fluorouracil pH 8.0, 37°C Plasmodium falciparum
2.4.2.10 0.0705
-
pyrazofurin pH 8.0, 37°C Plasmodium falciparum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.2.10 Plasmodium falciparum calculated
-
7.1