Literature summary extracted from
Rubio, L.M.; Flores, E.; Herrero, A.
Purification, cofactor analysis, and site-directed mutagenesis of Synechococcus ferredoxin-nitrate reductase (2002), Photosynth. Res., 72, 13-26.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.7.7.2 |
expression in Escherichia coli |
Synechococcus sp. |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.7.7.2 |
C12A |
no actiivty, no iron-sulfur cluster |
Synechococcus sp. |
1.7.7.2 |
C16S |
1.9% of wild type activity with ferredoxin, no iron-sulfur cluster |
Synechococcus sp. |
1.7.7.2 |
C56A |
no activity, no iron-sulfur cluster |
Synechococcus sp. |
1.7.7.2 |
C56S |
70% of wild type activity with ferredoxin, contains an iron-sulfur cluster |
Synechococcus sp. |
1.7.7.2 |
C9A |
no activity, no iron-sulfur cluster |
Synechococcus sp. |
1.7.7.2 |
C9H |
no narB expression |
Synechococcus sp. |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.7.7.2 |
Iron |
enzyme contains 1 [3Fe-4S] cluster |
Synechococcus sp. |
|
1.7.7.2 |
Molybdenum |
1 atom of Mo and 2 molecules of ribonucleotide-conjugated molybdopterin per molecule of protein |
Synechococcus sp. |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.7.7.2 |
nitrate + reduced ferredoxin |
Synechococcus sp. |
- |
nitrite + H2O + oxidized ferredoxin |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.7.7.2 |
Synechococcus sp. |
P39458 |
strain PCC 7942 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.7.7.2 |
recombinant enzyme |
Synechococcus sp. |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
1.7.7.2 |
-20°C, 20% glycerol, several months, no loss of activity |
Synechococcus sp. |
1.7.7.2 |
4°C, several weeks, no loss of activity |
Synechococcus sp. |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.7.7.2 |
nitrate + reduced ferredoxin |
- |
Synechococcus sp. |
nitrite + H2O + oxidized ferredoxin |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.7.7.2 |
narB |
- |
Synechococcus sp. |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.7.7.2 |
molybdopterin |
bis-molybdopterin guanine dinucleotide |
Synechococcus sp. |
|