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Literature summary extracted from

  • Yoshida, H.; Hensgens, C.M.; van der Laan, J.M.; Sutherland, J.D.; Hart, D.J.; Dijkstra, B.W.
    An approach to prevent aggregation during the purification and crystallization of wild type acyl coenzyme A: isopenicillin N acyltransferase from Penicillium chrysogenum (2005), Protein Expr. Purif., 41, 61-67.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.164 expression in Escherichia coli Penicillium chrysogenum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.164
-
 Penicillium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.164 11000
-
 1 * 11000 + 1 * 29000, SDS-PAGE Penicillium chrysogenum
2.3.1.164 29000
-
 1 * 11000 + 1 * 29000, SDS-PAGE Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.164 Penicillium chrysogenum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.164 recombinant wild-type enzyme with a C-terminal His-tag is purified in a single step using Ni2+ affinity chelate chromatography. A combination of 5 mM DTT + 250 mM NaCl + 5 mM EDTA is effective in preventing aggregation of the enzyme Penicillium chrysogenum

Subunits

EC Number Subunits Comment Organism
2.3.1.164 dimer 1 * 11000 + 1 * 29000, SDS-PAGE Penicillium chrysogenum

Synonyms

EC Number Synonyms Comment Organism
2.3.1.164 acyl coenzyme A: isopenicillin N acyltransferase
-
 Penicillium chrysogenum