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Literature summary extracted from

  • Bar, D.; Golbik, R.; Hubner, G.; Lilie, H.; Muller, E.C.; Naumann, M.; Otto, A.; Reuter, R.; Breunig, K.D.; Kriegel, T.M.
    The unique hexokinase of Kluyveromyces lactis. Molecular and functional characterization and evaluation of a role in glucose signaling (2003), J. Biol. Chem., 278, 39280-39286.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.1 gene RAG5, overexpression in enzyme-deficient strain JA6DELTArag5 Kluyveromyces lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 additional information inactivation by autophosphorylation at Ser157 utilizing MgATP2-, first order kinetics reaveal an extrapolated residual activity of about 60% Kluyveromyces lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 additional information
-
 additional information stopped-flow steady-state kinetics, dissociation constants of the monomer-homodimer equilibria Kluyveromyces lactis
2.7.1.1 0.196
-
 D-glucose pH 7.4, 25°C, recombinant enzyme at 0.001 mg/ml Kluyveromyces lactis
2.7.1.1 0.226
-
 ATP pH 7.4, 25°C, recombinant enzyme at 0.001 mg/ml Kluyveromyces lactis
2.7.1.1 0.39
-
 ATP pH 7.4, 25°C, recombinant enzyme at 1.0 mg/ml Kluyveromyces lactis
2.7.1.1 0.451
-
 D-glucose pH 7.4, 25°C, recombinant enzyme at 1.0 mg/ml Kluyveromyces lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.1 Mg2+
-
 Kluyveromyces lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 53476
-
 1 * 54000, recombinant enzyme, SDS-PAGE and sedimentation equilibrium, 1 * 53476, recombinant enzyme, mass spectrometry and sedimentation equilibrium Kluyveromyces lactis
2.7.1.1 53476
-
 2 * 54000, recombinant enzyme, SDS-PAGE and sedimentation equilibrium, 2 * 53476, recombinant enzyme, mass spectrometry and sedimentation equilibrium Kluyveromyces lactis
2.7.1.1 54000
-
 1 * 54000, recombinant enzyme, SDS-PAGE and sedimentation equilibrium, 1 * 53476, recombinant enzyme, mass spectrometry and sedimentation equilibrium Kluyveromyces lactis
2.7.1.1 54000
-
 2 * 54000, recombinant enzyme, SDS-PAGE and sedimentation equilibrium, 2 * 53476, recombinant enzyme, mass spectrometry and sedimentation equilibrium Kluyveromyces lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.1 ATP + D-glucose Kluyveromyces lactis
-
 ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 additional information Kluyveromyces lactis the enzyme is the only glucose and fructose phosphorylating enzyme, which is upregulating glucose transport and also mediating glucose repression ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Kluyveromyces lactis P33284 crabtree-negative yeast, gene RAG5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 recombinant enzyme from enzyme-deficient strain JA6DELTArag5, 25fold by anion exchange chromatography and ammonium sulfate fractionation Kluyveromyces lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 additional information
-
-
 Kluyveromyces lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + D-glucose
-
 Kluyveromyces lactis ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 additional information the enzyme is the only glucose and fructose phosphorylating enzyme, which is upregulating glucose transport and also mediating glucose repression Kluyveromyces lactis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.1 dimer 2 * 54000, recombinant enzyme, SDS-PAGE and sedimentation equilibrium, 2 * 53476, recombinant enzyme, mass spectrometry and sedimentation equilibrium Kluyveromyces lactis
2.7.1.1 monomer 1 * 54000, recombinant enzyme, SDS-PAGE and sedimentation equilibrium, 1 * 53476, recombinant enzyme, mass spectrometry and sedimentation equilibrium Kluyveromyces lactis
2.7.1.1 More dissociation constants of the monomer-homodimer equilibria, hydrodynamic Kluyveromyces lactis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.1 KlHxk1
-
 Kluyveromyces lactis
2.7.1.1 Rag5p
-
 Kluyveromyces lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.1 25
-
 assay at Kluyveromyces lactis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.1 additional information
-
 additional information
-
 Kluyveromyces lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 7.4
-
 assay at Kluyveromyces lactis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP
-
 Kluyveromyces lactis