EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.31 | glucose 6-phosphate | - |
Zea mays | |
4.1.1.31 | glycine | - |
Zea mays |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.31 | expression in Escherichia coli BL21(DE3)pLysS | Zea mays |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.31 | D228N | reduced apparent affinity for the activator glycine | Zea mays |
4.1.1.31 | E229A | maximal activation caused by glycine is greatly reduced, significantly lowered sensitivity to the inhibitors malate and aspartate. K(0.5) for phosphoenolpyruvate is lower than wild-type value | Zea mays |
4.1.1.31 | R226Q | maximal activation caused by glycine is greatly reduced, significantly lowered sensitivity to the inhibitors malate and aspartate. K(0.5) for phosphoenolpyruvate is significantly higher than that of wild-type enzyme | Zea mays |
4.1.1.31 | R231A | decreased apparent affinity for the activator glucose 6-phosphate | Zea mays |
4.1.1.31 | R232Q | decreased apparent affinity for the activator glucose 6-phosphate, reduced apparent affinity for the activator glycine | Zea mays |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.31 | aspartate | - |
Zea mays | |
4.1.1.31 | malate | - |
Zea mays |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.31 | Zea mays | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.31 | - |
Zea mays |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.31 | phosphoenolpyruvate + HCO3- | - |
Zea mays | phosphate + oxaloacetate | - |
? |