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Literature summary extracted from

  • Chou, W.I.; Pai, T.W.; Liu, S.H.; Hsiung, B.K.; Chang, M.D.
    The family 21 carbohydrate-binding module of glucoamylase from Rhizopus oryzae consists of two sites playing distinct roles in ligand binding (2006), Biochem. J., 396, 469-477.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.3 industry the enzyme is useful in industrial applications as glycoside hydrolase Rhizopus arrhizus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.3 W47A site-directed mutagenesis, the mutant shows altered kinetics and starch binding compared to the wild-type enzyme Rhizopus arrhizus
3.2.1.3 Y32A site-directed mutagenesis, the mutant shows altered kinetics and starch binding compared to the wild-type enzyme Rhizopus arrhizus
3.2.1.3 Y32A/Y47A site-directed mutagenesis, the mutant shows altered kinetics and starch binding compared to the wild-type enzyme Rhizopus arrhizus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 additional information ligand binding kinetics, wild-type enzyme and mutants Rhizopus arrhizus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 starch + H2O Rhizopus arrhizus
-
 starch + beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Rhizopus arrhizus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.3 (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-1-alpha-D-glucopyranose + beta-D-glucopyranose structure-function relationship, ligand binding Rhizopus arrhizus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 alpha-cyclodextrin + H2O
-
 Rhizopus arrhizus alpha-cyclodextrin + beta-D-glucose
-
 ?
3.2.1.3 beta-cyclodextrin + H2O
-
 Rhizopus arrhizus beta-cyclodextrin + beta-D-glucose
-
 ?
3.2.1.3 gamma-cyclodextrin + H2O
-
 Rhizopus arrhizus gamma-cyclodextrin + beta-D-glucose
-
 ?
3.2.1.3 isomaltotriose + H2O
-
 Rhizopus arrhizus ? + beta-D-glucose
-
 ?
3.2.1.3 maltoheptaose + H2O
-
 Rhizopus arrhizus maltohexaose + D-glucose
-
 ?
3.2.1.3 maltohexaose + H2O
-
 Rhizopus arrhizus maltopentaose + D-glucose
-
 ?
3.2.1.3 maltopentaose + H2O
-
 Rhizopus arrhizus maltotetraose + beta-D-glucose
-
 ?
3.2.1.3 maltotriose + H2O
-
 Rhizopus arrhizus maltose + beta-D-glucose
-
 ?
3.2.1.3 starch + H2O
-
 Rhizopus arrhizus starch + beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 More secondary structure analysis, the enzyme contains a family 21 carbohydrate-binding module involving residues W47, Y83, and Y93, the enzyme consists of a catalytic domain and a starch binding domain connected by an O-glycosylated peptide linker located at the N-terminus, molecular modelling, structure-function relatioship Rhizopus arrhizus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 GA
-
 Rhizopus arrhizus
3.2.1.3 glucoamylase
-
 Rhizopus arrhizus
3.2.1.3 More the enzyme belongs to the glycohydrolase family 21 Rhizopus arrhizus