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Literature summary extracted from

  • Thorsen, T.S.; Johnsen, A.H.; Josefsen, K.; Jensen, B.
    Identification and characterization of glucoamylase from the fungus Thermomyces lanuginosus (2006), Biochim. Biophys. Acta, 1764, 671-676.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.3 gene gla1, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris, phylogenetic analysis Thermomyces lanuginosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 3.5
-
 maltose pH 5.0, 70°C Thermomyces lanuginosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 66000
-
 x * 66000, SDS-PAGE Thermomyces lanuginosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Thermomyces lanuginosus Q58HN1 precursor; gene gla1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 amylopectin + H2O 94% of activity with amylose Thermomyces lanuginosus amylopectin + beta-D-glucose
-
 ?
3.2.1.3 amylose + H2O preferred substrate Thermomyces lanuginosus amylose + beta-D-glucose
-
 ?
3.2.1.3 glycogen + H2O 87% of activity with amylose Thermomyces lanuginosus glycogen + beta-D-glucose
-
 ?
3.2.1.3 maltose + H2O 14% of activity with amylose Thermomyces lanuginosus beta-D-glucose + D-glucose
-
 ?
3.2.1.3 maltotriose + H2O 40% of activity with amylose Thermomyces lanuginosus maltose + beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 ? x * 66000, SDS-PAGE Thermomyces lanuginosus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 GA
-
 Thermomyces lanuginosus
3.2.1.3 glucoamylase
-
 Thermomyces lanuginosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 70
-
-
 Thermomyces lanuginosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.3 25.3
-
 maltose pH 5.0, 70°C Thermomyces lanuginosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 5
-
-
 Thermomyces lanuginosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.3 Thermomyces lanuginosus isoelectric focusing
-
 3.84