EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | additional information | - |
according to its CD spectrum, beta-galactosidase is mainly a beta-type protein. The antiparallel beta-sheet appears as the amino type of regular conformation (25%) | Kluyveromyces lactis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.23 | lactose + H2O | Kluyveromyces lactis | - |
D-glucose + D-galactose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.23 | Kluyveromyces lactis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.23 | - |
Kluyveromyces lactis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.23 | commercial preparation | - |
Kluyveromyces lactis | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 1709 | - |
purified commercial preparation | Kluyveromyces lactis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.23 | 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Kluyveromyces lactis | 2-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.23 | lactose + H2O | - |
Kluyveromyces lactis | D-glucose + D-galactose | - |
? | |
3.2.1.23 | additional information | structure-activity relationship as a function of the pH, overview | Kluyveromyces lactis | ? | - |
? | |
3.2.1.23 | o-nitrophenyl-beta-D-galactoside + H2O | - |
Kluyveromyces lactis | o-nitrophenol + beta-D-galactose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.23 | More | secondary structure by circular dichroism, the enzyme is a beta-type protein, having 22% beta-turns, 14% parallel beta-sheet, 25% antiparallel beta-sheet,34% unordered structure, and only 5% alpha-helix, structure-function relationship, highest secondary structure content at pH 6.5-7.0, overview | Kluyveromyces lactis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.23 | lactase | - |
Kluyveromyces lactis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 37 | - |
assay at | Kluyveromyces lactis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 7.5 | - |
structure-activity relationship as a function of the pH, overview | Kluyveromyces lactis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 5.5 | 8.5 | - |
Kluyveromyces lactis |