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Literature summary extracted from
- Purification and characterization of a new type of serine carboxypeptidase from Monascus purpureus (2004), J. Ind. Microbiol. Biotechnol., 31, 23-28.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.16.5 | 2-mercaptoethanol | 1 mM, 10% inhibition | Monascus purpureus |  |
| 3.4.16.5 | CaCl2 | 1 mM, 16% inhibition | Monascus purpureus | |
| 3.4.16.5 | chymostatin | 1 mM, 89% inhibition | Monascus purpureus | |
| 3.4.16.5 | CuCl | 1 mM, 37% inhibition | Monascus purpureus | |
| 3.4.16.5 | CuCl2 | 1 mM, 39% inhibition | Monascus purpureus | |
| 3.4.16.5 | diisopropylfluorophosphate | 1 mM, 63% inhibition | Monascus purpureus | |
| 3.4.16.5 | FeCl3 | 1 mM, 92% inhibition | Monascus purpureus | |
| 3.4.16.5 | HgCl2 | 1 mM, 54% inhibition | Monascus purpureus | |
| 3.4.16.5 | MnCl2 | 1 mM, 14% inhibition | Monascus purpureus | |
| 3.4.16.5 | Nalpha-tosyl-L-Lys-chloromethyl ketone | 1 mM, 13% inhibition | Monascus purpureus | |
| 3.4.16.5 | Nalpha-tosyl-L-Phe-chloromethyl ketone | 1 mM, 57% inhibition | Monascus purpureus | |
| 3.4.16.5 | NEM | 1 mM, 71% inhibition | Monascus purpureus | |
| 3.4.16.5 | PbCl2 | 1 mM, 95% inhibition | Monascus purpureus | |
| 3.4.16.5 | PCMB | 1 mM, 95% inhibition | Monascus purpureus | |
| 3.4.16.5 | pepstatin A | 1 mM, 8% inhibition | Monascus purpureus | |
| 3.4.16.5 | Phenylmethylsulfonylfluoride | 1 mM, 72% inhibition | Monascus purpureus | |
| 3.4.16.5 | piperastatin A | 1 mM, 70% inhibition | Monascus purpureus | |
| 3.4.16.5 | SDS | 1 mM, complete inhibition | Monascus purpureus | |
| 3.4.16.5 | SnCl2 | 1 mM, complete inhibition | Monascus purpureus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.16.5 | 0.86 | - |
benzyloxycarbonyl-Tyr-Glu | pH 4.0, 37°C | Monascus purpureus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.16.5 | 64000 | - |
1 * 64000 + 1 * 67000, SDS-PAGE | Monascus purpureus |
| 3.4.16.5 | 67000 | - |
1 * 64000 + 1 * 67000, SDS-PAGE | Monascus purpureus |
| 3.4.16.5 | 132000 | - |
gel filtration | Monascus purpureus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.16.5 | Monascus purpureus | - |
IFO 4478 | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.16.5 | glycoprotein | contains 17% carbohydrate | Monascus purpureus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.16.5 | - |
Monascus purpureus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.16.5 | 2.13 | - |
- |
Monascus purpureus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.16.5 | benzyloxycarbonyl-Ala-Glu + H2O | 76% of the activity with benzyloxycarbonyl-Glu-Tyr | Monascus purpureus | benzyloxycarbonyl-Ala + Glu | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Glu-Phe + H2O | 119% of the activity with benzyloxycarbonyl-Glu-Tyr | Monascus purpureus | benzyloxycarbonyl-Glu + Phe | - |
? | |
| 3.4.16.5 | Benzyloxycarbonyl-Glu-Tyr + H2O | - |
Monascus purpureus | Benzyloxycarbonyl-Glu + Tyr | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Gly-Pro-Leu-Gly + H2O | 59% of the activity with benzyloxycarbonyl-Glu-Tyr | Monascus purpureus | benzyloxycarbonyl-Gly-Pro-Leu + Gly | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Phe-Ala + H2O | 77% of the activity with benzyloxycarbonyl-Glu-Tyr | Monascus purpureus | benzyloxycarbonyl-Phe + Ala | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Phe-Gly + H2O | 24% of the activity with benzyloxycarbonyl-Glu-Tyr | Monascus purpureus | benzyloxycarbonyl-Phe + Gly | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Phe-Leu + H2O | 21% of the activity with benzyloxycarbonyl-Glu-Tyr | Monascus purpureus | benzyloxycarbonyl-Phe + Leu | - |
? | |
| 3.4.16.5 | Benzyloxycarbonyl-Phe-Tyr + H2O | 18% of the activity with benzyloxycarbonyl-Glu-Tyr | Monascus purpureus | Benzyloxycarbonyl-Phe + Tyr | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Tyr-Glu + H2O | 134% of the activity with benzyloxycarbonyl-Glu-Tyr | Monascus purpureus | benzyloxycarbonyl-Tyr + Glu | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.16.5 | dimer | 1 * 64000 + 1 * 67000, SDS-PAGE | Monascus purpureus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.16.5 | 40 | - |
hydrolysis of benzyloxycarbonyl-Glu-Tyr | Monascus purpureus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.16.5 | 50 | - |
pH 5.0, 15 min, stable up to | Monascus purpureus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.16.5 | 291 | - |
benzyloxycarbonyl-Tyr-Glu | pH 4.0, 37°C | Monascus purpureus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.16.5 | 4 | - |
hydrolysis of benzyloxycarbonyl-Glu-Tyr | Monascus purpureus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.16.5 | 2 | 8 | 37°C, 1 h, stable | Monascus purpureus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.4.16.5 | Monascus purpureus | - |
- |
3.7 |
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