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Literature summary extracted from

  • Herrera-Camacho, I.; Morales-Monterrosas, R.; Quiroz-Alvarez, R.
    Aminopeptidase yscCo-II: a new cobalt-dependent aminopeptidase from yeast-purification and biochemical characterization (2000), Yeast, 16, 219-229.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.24 1,10-phenanthroline 1 mM, 91% inhibition Saccharomyces cerevisiae
3.4.11.24 antipain 0.01 mg/ml, 93% inhibition Saccharomyces cerevisiae
3.4.11.24 bestatin 0.004 mg/ml, 94% inhibition Saccharomyces cerevisiae
3.4.11.24 Chloroquine 5 mM, 79% inhibition Saccharomyces cerevisiae
3.4.11.24 CrCl2 1 mM, 83% inhibition in presence of 1 mM CoCl2 Saccharomyces cerevisiae
3.4.11.24 CuCl2 1 mM, 96% inhibition in presence of 1 mM CoCl2 Saccharomyces cerevisiae
3.4.11.24 EDTA 5 mM, 90% inhibition Saccharomyces cerevisiae
3.4.11.24 HgCl2 5 mM, 98% inhibition in presence of 1 mM CoCl2 Saccharomyces cerevisiae
3.4.11.24 Leu-hydroxamate 1 mM, 73% inhibition Saccharomyces cerevisiae
3.4.11.24 leupeptin 0.01 mg/ml, 51% inhibition Saccharomyces cerevisiae
3.4.11.24 Lys-hydroxamate 1 mM, 81% inhibition Saccharomyces cerevisiae
3.4.11.24 MgSO4 1 mM, 24% inhibition in presence of 1 mM CoCl2 Saccharomyces cerevisiae
3.4.11.24 MnSO4 1 mM, 26% inhibition in presence of 1 mM CoCl2 Saccharomyces cerevisiae
3.4.11.24 NiSO4 1 mM, 40% inhibition in presence of 1 mM CoCl2 Saccharomyces cerevisiae
3.4.11.24 nitrilotriacetic acid 5 mM, 95% inhibition Saccharomyces cerevisiae
3.4.11.24 p-hydroxymercuribenzoate 5 mM, 90% inhibition Saccharomyces cerevisiae
3.4.11.24 phenylmethylsulfonyl fluoride 1 mM, 95% inhibition Saccharomyces cerevisiae
3.4.11.24 tosyl-lysine chloromethyl ketone 5 mM, 94% inhibition Saccharomyces cerevisiae
3.4.11.24 ZnCl2 1 mM, 70% inhibition in presence of 1 mM CoCl2 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.24 0.16
-
 Lys-4-nitroanilide 37°C, 0.5 mM CoCl2 Saccharomyces cerevisiae
3.4.11.24 0.16
-
 Arg-4-nitroanilide 37°C, 0.5 mM CoCl2 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.24 Co2+ activity is strictly dependent on, maximal activity at 0.5 mM CoCl2 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.24 48000
-
 6 * 48000, SDS-PAGE Saccharomyces cerevisiae
3.4.11.24 290000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.24 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.24
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.24 4.05
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
3.4.11.24 4°C, 50% loss of activity after 4 days, 80% loss of activity after 8 days. Remains active for 2 months, when concentrated and kept in a suspension with 4 M ammonium sulfate in 20 mM MOPS/Tris, pH 7 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.24 Arg-4-nitroanilide + H2O 105.2% of the activity with Lys-4-nitroanilide Saccharomyces cerevisiae Arg + 4-nitroaniline
-
 ?
3.4.11.24 Glu-4-nitroanilide + H2O 5.4% of the activity with Lys-4-nitroanilide Saccharomyces cerevisiae Glu + 4-nitroaniline
-
 ?
3.4.11.24 Leu-4-nitroanilide + H2O 12.2% of the activity with Lys-4-nitroanilide Saccharomyces cerevisiae Leu + 4-nitroaniline
-
 ?
3.4.11.24 Lys-4-nitroanilide + H2O
-
 Saccharomyces cerevisiae Lys + 4-nitroaniline
-
 ?
3.4.11.24 Pro-4-nitroanilide + H2O 11.7% of the activity with Lys-4-nitroanilide Saccharomyces cerevisiae Pro + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.24 hexamer 6 * 48000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.4.11.24 aminopeptidase yscCo-II
-
 Saccharomyces cerevisiae
3.4.11.24 APCo-II
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.24 7
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.24 6 8 pH 6: about 55% of maximal activity, pH 11: about 35% of maximal activity Saccharomyces cerevisiae