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Literature summary extracted from
- A coupled fluorescent assay for histone methyltransferases (2005), Anal. Biochem., 342, 86-92.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | molecular biology | a coupled fluorescent assay for histone methyltransferase utilizes S-adenosylhomocysteine hydrolase to hydrolyze the methyltransfer product S-adenosylhomocysteine to homocysteine and adenosine. The homocysteine concentration is then determined through conjugation of its free sulfhydryl moiety to a thiol-sensitive fluorophore. the assay allows rapid and facile determination of histine methyltransferase kinetics and can be adapted to measure the enzymatic activity of a wide variety of S-adenosylmethionine-dependent methyltransferases | Saccharolobus solfataricus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.13.2.1 | - |
Saccharolobus solfataricus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.13.2.1 | Saccharolobus solfataricus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.13.2.1 | - |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.2.1 | S-adenosyl-DL-homocysteine + H2O | - |
Saccharolobus solfataricus | DL-homocysteine + adenosine | - |
? |  |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | SAHH | - |
Saccharolobus solfataricus |
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Release 2023.1 (January 2023)
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