EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.2.7 | enzyme or cytochrome cL, hanging drop vapour diffusion method, 16°C, 0.001 ml of 20 mg/mL enzyme or cofactor in 40 mM Tris-HCl buffer, pH 7.5, is mixed with an equal volume of precipitant colution containing 0.2 M potassium thiocyanate and 20% PEG 3350 for the enzyme crystallization, and 2 mM ZnSO4, 20% PEG 10000, and 100 mM HEPES, pH 7.3, for the crystallization of Cyt cL, 1 week to 1 month, X-ray diffraction structure determination and analysis at 1.98-2.5 A resolution | Hyphomicrobium denitrificans |
1.1.2.7 | hanging-drop vapor diffusion method, X-ray structures of methanol dehydrogenase (Hd-MDH) and cytochrome cL at 2.5 A and 2.0 A resolution, respectively. Docking simulation between the coupled cytochrome cL molecules and the heterotetrameric methanol dehydrogenase | Hyphomicrobium denitrificans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.2.7 | additional information | - |
additional information | steady-state and stopped-flow kinetics | Hyphomicrobium denitrificans | |
1.1.2.7 | 0.0003 | - |
ferricytochrome cL | pH 5.5, ionic strength: 0.0160 M, steady-state kinetic parameter | Hyphomicrobium denitrificans | |
1.1.2.7 | 0.0006 | - |
ferricytochrome cL | pH 6.0, ionic strength: 0.0172 M, steady-state kinetic parameter | Hyphomicrobium denitrificans | |
1.1.2.7 | 0.0011 | - |
ferricytochrome cL | pH 6.5, ionic strength: 0.0183 M, steady-state kinetic parameter | Hyphomicrobium denitrificans | |
1.1.2.7 | 0.0026 | - |
ferricytochrome cL | pH 5.0, ionic strength: 0.0181 M, steady-state kinetic parameter | Hyphomicrobium denitrificans | |
1.1.2.7 | 0.009 | - |
ferricytochrome cL | pH 4.5, ionic strength: 0.0168 M, steady-state kinetic parameter | Hyphomicrobium denitrificans | |
1.1.2.7 | 0.105 | - |
ferricytochrome cL | pH 7.0, ionic strength: 0.0198 M, steady-state kinetic parameter | Hyphomicrobium denitrificans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.2.7 | 9000 | - |
2 * 65000 + 2 * 9000 | Hyphomicrobium denitrificans |
1.1.2.7 | 65000 | - |
2 * 65000 + 2 * 9000 | Hyphomicrobium denitrificans |
1.1.2.7 | 148000 | - |
- |
Hyphomicrobium denitrificans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.2.7 | methanol + 2 oxidized cytochrome cL | Hyphomicrobium denitrificans | way of electron transfer from methanol to O2, overview | formaldehyde + 2 reduced cytochrome cL | - |
? | |
1.1.2.7 | methanol + 2 oxidized cytochrome cL | Hyphomicrobium denitrificans A3151 | way of electron transfer from methanol to O2, overview | formaldehyde + 2 reduced cytochrome cL | - |
? | |
1.1.2.7 | methanol + ferricytochrome cL | Hyphomicrobium denitrificans | - |
formaldehyde + ferrocytochrome cL | - |
? | |
1.1.2.7 | methanol + ferricytochrome cL | Hyphomicrobium denitrificans A3151 | - |
formaldehyde + ferrocytochrome cL | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.2.7 | Hyphomicrobium denitrificans | - |
- |
- |
1.1.2.7 | Hyphomicrobium denitrificans A3151 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.2.7 | - |
Hyphomicrobium denitrificans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.2.7 | additional information | - |
- |
Hyphomicrobium denitrificans |
1.1.2.7 | 0.12 | 0.7 | dependent on pH, overview | Hyphomicrobium denitrificans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.2.7 | methanol + 2 oxidized cytochrome cL | - |
Hyphomicrobium denitrificans | formaldehyde + 2 reduced cytochrome cL | - |
? | |
1.1.2.7 | methanol + 2 oxidized cytochrome cL | way of electron transfer from methanol to O2, overview | Hyphomicrobium denitrificans | formaldehyde + 2 reduced cytochrome cL | - |
? | |
1.1.2.7 | methanol + 2 oxidized cytochrome cL | - |
Hyphomicrobium denitrificans A3151 | formaldehyde + 2 reduced cytochrome cL | - |
? | |
1.1.2.7 | methanol + 2 oxidized cytochrome cL | way of electron transfer from methanol to O2, overview | Hyphomicrobium denitrificans A3151 | formaldehyde + 2 reduced cytochrome cL | - |
? | |
1.1.2.7 | methanol + ferricytochrome cL | - |
Hyphomicrobium denitrificans | formaldehyde + ferrocytochrome cL | - |
? | |
1.1.2.7 | methanol + ferricytochrome cL | - |
Hyphomicrobium denitrificans A3151 | formaldehyde + ferrocytochrome cL | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.2.7 | tetramer | alpha2beta2 structure, subunit organization and structure, docking model, overview | Hyphomicrobium denitrificans |
1.1.2.7 | tetramer | 2 * 65000 + 2 * 9000 | Hyphomicrobium denitrificans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.2.7 | Hd-MDH | - |
Hyphomicrobium denitrificans |
1.1.2.7 | MDH | - |
Hyphomicrobium denitrificans |
1.1.2.7 | methanol dehydrogenase | - |
Hyphomicrobium denitrificans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.2.7 | 25 | - |
assay at | Hyphomicrobium denitrificans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.2.7 | additional information | - |
additional information | kcat at different pH, overview | Hyphomicrobium denitrificans | |
1.1.2.7 | 0.15 | - |
ferricytochrome cL | pH 5.5, ionic strength: 0.0160 M, steady-state kinetic parameter | Hyphomicrobium denitrificans | |
1.1.2.7 | 0.21 | - |
ferricytochrome cL | pH 6.0, ionic strength: 0.0172 M, steady-state kinetic parameter | Hyphomicrobium denitrificans | |
1.1.2.7 | 0.33 | - |
ferricytochrome cL | pH 6.5, ionic strength: 0.0183 M, steady-state kinetic parameter | Hyphomicrobium denitrificans | |
1.1.2.7 | 0.41 | - |
ferricytochrome cL | pH 5.0, ionic strength: 0.0181 M, steady-state kinetic parameter | Hyphomicrobium denitrificans | |
1.1.2.7 | 0.45 | - |
ferricytochrome cL | pH 4.5, ionic strength: 0.0168 M, steady-state kinetic parameter | Hyphomicrobium denitrificans | |
1.1.2.7 | 0.87 | - |
methanol | pH 7.0, 25°C | Hyphomicrobium denitrificans | |
1.1.2.7 | 0.87 | - |
ferricytochrome cL | pH 7.0, ionic strength: 0.0198 M, steady-state kinetic parameter | Hyphomicrobium denitrificans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.2.7 | 7 | - |
- |
Hyphomicrobium denitrificans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.2.7 | 7 | 9 | the enzyme oxidation step becomes rate-limiting at pH 9.0 | Hyphomicrobium denitrificans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.2.7 | cytochrome cL | - |
Hyphomicrobium denitrificans | |
1.1.2.7 | pyrroloquinoline quinone | enzyme contains two semiquinone pyrroloquinoline quinone groups per heterotetramer | Hyphomicrobium denitrificans |