EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.99.2 | expressed in Escherichia coli | Pseudomonas aeruginosa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.4.10 | C139S | no activity | Pseudomonas aeruginosa |
1.8.4.10 | C140S | mutant enzyme shows 3% of wild-type activity | Pseudomonas aeruginosa |
1.8.4.10 | C228S | no activity | Pseudomonas aeruginosa |
1.8.4.10 | C231S | no activity | Pseudomonas aeruginosa |
1.8.4.10 | C256S | no activity | Pseudomonas aeruginosa |
1.8.99.2 | C139S | no detectable activity | Pseudomonas aeruginosa |
1.8.99.2 | C140S | 3% activity of the wild type enzyme | Pseudomonas aeruginosa |
1.8.99.2 | C228S | no detectable activity | Pseudomonas aeruginosa |
1.8.99.2 | C231S | no detectable activity | Pseudomonas aeruginosa |
1.8.99.2 | C256S | no detectable activity | Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.99.2 | 2.7 | - |
thioredoxin | thioredoxin originating from Clamydomonas reinhardtii | Pseudomonas aeruginosa | |
1.8.99.2 | 8.6 | - |
thioredoxin | thioredoxin originating from Escherichia coli | Pseudomonas aeruginosa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.99.2 | 30470 | - |
MALDI-TOF mass spectrometry, the enzyme used is a recombinant protein with 48 additional residues containing the His6 tag | Pseudomonas aeruginosa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.4.10 | Pseudomonas aeruginosa | - |
- |
- |
1.8.99.2 | Pseudomonas aeruginosa | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.8.99.2 | 0.302 | - |
mutant enzyme C140S, at 30°C in 100 mM Tris-HCl buffer pH 8.0 containing 1 mM EDTA, 5 mM DTT, 0.106 mM thioredoxin from Escherichia coli, and 0.05 mM APS | Pseudomonas aeruginosa |
1.8.99.2 | 10.3 | - |
wild type enzyme, at 30°C in 100 mM Tris-HCl buffer pH 8.0 containing 1 mM EDTA, 5 mM DTT, 0.106 mM thioredoxin from Escherichia coli, and 0.05 mM APS | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.99.2 | adenylylsulfate + thioredoxin | - |
Pseudomonas aeruginosa | AMP + sulfite + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.99.2 | monomer | SDS-PAGE | Pseudomonas aeruginosa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.4.10 | PaAPR | - |
Pseudomonas aeruginosa |
1.8.99.2 | 5'-adenylylsulfate reductase | - |
Pseudomonas aeruginosa |
1.8.99.2 | APR | - |
Pseudomonas aeruginosa |