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Literature summary extracted from

  • Yamamoto, T.; Miyazaki, K.; Eguchi, T.
    Substrate specificity analysis and inhibitor design of homoisocitrate dehydrogenase (2007), Bioorg. Med. Chem., 15, 1346-1355.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.87 expression in Escherichia coli Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.87 (R,E)-(3-carboxypropylidene)malic acid competitive Deinococcus radiodurans
1.1.1.87 (R,E)-(3-carboxypropylidene)malic acid competitive Saccharomyces cerevisiae
1.1.1.87 (R,E)-(3-hydroxypropylidene)malic acid competitive Deinococcus radiodurans
1.1.1.87 (R,E)-(3-hydroxypropylidene)malic acid competitive Saccharomyces cerevisiae
1.1.1.87 (R,Z)-(3-carboxypropylidene)malic acid competitive Deinococcus radiodurans
1.1.1.87 (R,Z)-(3-carboxypropylidene)malic acid competitive Saccharomyces cerevisiae
1.1.1.87 (R,Z)-(3-hydroxypropylidene)malic acid competitive Deinococcus radiodurans
1.1.1.87 (R,Z)-(3-hydroxypropylidene)malic acid competitive Saccharomyces cerevisiae
1.1.1.87 3-vinylmalate
-
 Deinococcus radiodurans
1.1.1.87 additional information synthesis and inhibition potency of substrate analogue inhibitors derived from 3-hydroxyalkylidene- and 3-carboxyalkylidenemalate derivatives, overview Deinococcus radiodurans
1.1.1.87 additional information synthesis and inhibition potency of substrate analogue inhibitors derived from 3-hydroxyalkylidene- and 3-carboxyalkylidenemalate derivatives, overview, no inhibition by 3-vinylmalate Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.87 0.018
-
 homoisocitrate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 0.083
-
 3-vinylmalate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 0.211
-
 homoisocitrate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 0.29
-
 (2R,3S)-3-propylmalic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 0.291
-
 isocitrate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 0.41
-
 (2R,3S)-3-allylmalic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 0.44
-
 (2R,3S)-3-(4-hydroxybutyl)malate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 0.47
-
 (2R,3S)-3-(4-hydroxybutyl)malate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 0.51
-
 (2R,3S)-3-(3-hydroxypropyl)malate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 0.58
-
 (2R,3S)-3-(3-hydroxypropyl)malate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 0.59
-
 (2R,3S)-3-(2-hydroxyethyl)malate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 0.6
-
 ethylmalate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 0.78
-
 (2R,3S)-3-propylmalic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 1
-
 ethylmalate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 1.33
-
 isopropylmalate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 2.1
-
 (2R,3S)-3-(2-hydroxyethyl)malate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 2.7
-
 (2R,3S)-3-(4-pentenyl)malic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 2.8
-
 (2R,3S)-3-allylmalic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 3.4
-
 3-vinylmalate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 9.4
-
 (2R,3S)-3-(4-pentenyl)malic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.87 Mg2+
-
 Saccharomyces cerevisiae
1.1.1.87 Mg2+
-
 Deinococcus radiodurans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.87 Deinococcus radiodurans
-
-
-
1.1.1.87 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.87 recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.87 additional information
-
 substrate specificity, recombinant enzyme, overview Saccharomyces cerevisiae
1.1.1.87 additional information
-
 substrate specificity, recombinant enzyme, overview Deinococcus radiodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.87 (2R,3S)-3-(2-hydroxyethyl)malate + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
1.1.1.87 (2R,3S)-3-(2-hydroxyethyl)malate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
1.1.1.87 (2R,3S)-3-(3-aminopropyl)malate + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
1.1.1.87 (2R,3S)-3-(3-aminopropyl)malate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
1.1.1.87 (2R,3S)-3-(3-hydroxypropyl)malate + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
1.1.1.87 (2R,3S)-3-(3-hydroxypropyl)malate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
1.1.1.87 (2R,3S)-3-(4-hydroxybutyl)malate + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
1.1.1.87 (2R,3S)-3-(4-hydroxybutyl)malate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
1.1.1.87 (2R,3S)-3-(4-pentenyl)malic acid + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
1.1.1.87 (2R,3S)-3-(4-pentenyl)malic acid + NAD+
-
 Deinococcus radiodurans ?
-
 ?
1.1.1.87 (2R,3S)-3-allylmalic acid + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
1.1.1.87 (2R,3S)-3-allylmalic acid + NAD+
-
 Deinococcus radiodurans ?
-
 ?
1.1.1.87 (2R,3S)-3-propylmalic acid + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
1.1.1.87 (2R,3S)-3-propylmalic acid + NAD+
-
 Deinococcus radiodurans ?
-
 ?
1.1.1.87 3-vinylmalate + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
1.1.1.87 3-vinylmalate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
1.1.1.87 ethylmalate + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
1.1.1.87 ethylmalate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
1.1.1.87 homoisocitrate + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
1.1.1.87 homoisocitrate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
1.1.1.87 isocitrate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
1.1.1.87 isopropylmalate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
1.1.1.87 additional information no activity with isocitrate, isopropylmalate, and tartrate Saccharomyces cerevisiae ?
-
 ?
1.1.1.87 additional information no activity with tartrate Deinococcus radiodurans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.87 HICDH
-
 Saccharomyces cerevisiae
1.1.1.87 HICDH
-
 Deinococcus radiodurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.87 28
-
 assay at Deinococcus radiodurans
1.1.1.87 36
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.87 0.14
-
 (2R,3S)-3-(4-pentenyl)malic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 0.34
-
 (2R,3S)-3-allylmalic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 0.37
-
 isopropylmalate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 0.5
-
 3-vinylmalate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 0.61
-
 ethylmalate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 0.7
-
 (2R,3S)-3-propylmalic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 0.77
-
 3-vinylmalate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 0.78
-
 (2R,3S)-3-(2-hydroxyethyl)malate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 2 8 (2R,3S)-3-(4-hydroxybutyl)malate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 2.1
-
 (2R,3S)-3-(4-hydroxybutyl)malate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 3
-
 (2R,3S)-3-(4-pentenyl)malic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 4.8
-
 (2R,3S)-3-(3-hydroxypropyl)malate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 17
-
 homoisocitrate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 25
-
 (2R,3S)-3-(2-hydroxyethyl)malate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 29
-
 (2R,3S)-3-(3-hydroxypropyl)malate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 37
-
 (2R,3S)-3-allylmalic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 43
-
 isocitrate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 43
-
 ethylmalate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 46
-
 homoisocitrate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 53
-
 (2R,3S)-3-propylmalic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.87 7.8
-
 assay at Saccharomyces cerevisiae
1.1.1.87 7.8
-
 assay at Deinococcus radiodurans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.87 NAD+
-
 Saccharomyces cerevisiae
1.1.1.87 NAD+
-
 Deinococcus radiodurans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.87 0.072
-
 (R,Z)-(3-carboxypropylidene)malic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 0.088
-
 3-vinylmalate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 0.26
-
 (R,Z)-(3-carboxypropylidene)malic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 0.51
-
 (R,Z)-(3-hydroxypropylidene)malic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 0.79
-
 (R,E)-(3-carboxypropylidene)malic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 1.4
-
 (R,E)-(3-hydroxypropylidene)malic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.1.1.87 3.1
-
 (R,E)-(3-carboxypropylidene)malic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 5.2
-
 (R,E)-(3-hydroxypropylidene)malic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.1.1.87 15.3
-
 (R,Z)-(3-hydroxypropylidene)malic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans